EGFR-IN-108 chloride (Compound Ru3S) is an EGFR inhibitor with IC50 of 5.8 nM for hEGFR. EGFR-IN-108 chloride can induce apoptosis and has antiproliferative activity against cancer cells. EGFR-IN-108 chloride also has anti-angiogenic effects.

Physicochemical Properties

Molecular Formula	C45H40CL3F3N8O3RU
Molecular Weight	1005.28
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Ru4S (0.2-100 μM; 48 h) is cytotoxic to HepG2, Caco-2, HT-29, MCF-7, A549, and HEK293T cell lines with IC50 values of 23.76, 2.47, 6.24, 5.35, 29.90, and 33.15 μM, respectively[1]. Ru4S (0.78-50 μM; 48 h) induces apoptosis in HepG2 cells[1].
Cell Assay	Cell Cytotoxicity Assay[1] Cell Types: HepG2, Caco-2, HT-29, MCF-7, A549, HEK293T cells Tested Tested Concentrations: 0.2-100 μM Incubation Duration: 48 h Experimental Results: exhibited cytotoxicity. Cell Proliferation Assay[1] Cell Types: HepG2 cells Tested Tested Concentrations: 0.78, 3.12, 12.5, and 50 μM Incubation Duration: 48 h Experimental Results: increased Sub G1 (p < 0.0001) and decreased G0/G1, S, and G2/M percentages (p < 0.05) at 0.78 μM. And slightly increased Sub G1 (p < 0.01), slightly decreased G0/G1 (p < 0.01), and decreased G2/M (p < 0.05) at 3.12 and 12.5 μM. Increased Sub G1(p < 0.0001) and decreased S (p < 0.01), and did not affect the G0/G1 and G2/M phases at 50 μM.
References	[1]. Synthesis of Sorafenib-Ruthenium Complexes, Investigation of Biological Activities and Applications in Drug Delivery Systems as an Anticancer Agent. J Med Chem. 2024 Mar 12.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	0.9947 mL	4.9737 mL	9.9475 mL
	5 mM	0.1989 mL	0.9947 mL	1.9895 mL
	10 mM	0.0995 mL	0.4974 mL	0.9947 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.