1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide HCl is a carbodiimide reagent that can form nucleic acids and compounds with amide bonds. 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide HCl accelerates the formation reactions of esters, amides and peptides. As a condensation agent and dehydrating agent, it is often used in polynucleotide synthesis, hydroxylation, lactonization and esterification.

Physicochemical Properties

Molecular Formula	C8H18CLN3
Molecular Weight	191.7016
Exact Mass	191.118
CAS #	25952-53-8
PubChem CID	2723939
Appearance	White to off-white solid powder
Density	0.877 g/mL at 20 °C(lit.)
Boiling Point	269.1ºC at 760 mmHg
Melting Point	110-115 °C(lit.)
Flash Point	107.9ºC
Vapour Pressure	0.171mmHg at 25°C
Index of Refraction	n20/D 1.461
LogP	1.933
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	5
Heavy Atom Count	12
Complexity	134
Defined Atom Stereocenter Count	0
InChi Key	FPQQSJJWHUJYPU-UHFFFAOYSA-N
InChi Code	InChI=1S/C8H17N3.ClH/c1-4-9-8-10-6-5-7-11(2)3;/h4-7H2,1-3H3;1H
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is an extremely helpful chemical for forming amide bonds, or peptide bonds, in aqueous fluids [5]. In order to prepare fatty amines for HPLC and polyaniline-carbon nanotubes for cryogenic biosensors, 1-(3-Dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) is frequently utilized. The experiments include pre-column derivatization, solid-phase microregulation of peptides, preparation and detection of calcium sensors, creation of molecular beacons for DNA research, and simulation of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride (EDC.HCl) using fluorometric measurement [2]. Acid decarboxylase (GAD) does not significantly limit substrate degradation [3].

References

[1]. Spectrophotometric determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow injection analysis. Anal Sci. 2008 Apr;24(4):505-8. [2]. Determination of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride by flow-injection analysis based on a specific condensation reaction between malonic acid and ethylenediamine. Anal Sci. 2009 Mar;25(3):389-93. [3]. AMADH inhibitor optimization and its effects on GABA accumulation in soybean sprouts under NaCl-CaCl 2 treatment. 3 Biotech. 2019 May;9(5):184.

Solubility Data

Solubility (In Vitro)

H2O : ~100 mg/mL (~521.65 mM) DMSO : ~62.5 mg/mL (~326.03 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (13.04 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.2165 mL	26.0824 mL	52.1648 mL
	5 mM	1.0433 mL	5.2165 mL	10.4330 mL
	10 mM	0.5216 mL	2.6082 mL	5.2165 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.