EC17 sodium (Folate-FITC) is a fluorescein isothiocyanate (FITC) conjugated folic acid with potential antineoplastic activity. It also acts as a FRα-targeting agent that fluoresces at 500nm. EC17 is conjugate consisting of fluorescein isothiocyanate (FITC) conjugated with folate with potential antineoplastic activity. Folate-FITC binds to folate receptors, which are overexpressed on the surfaces of many cancer cells including kidney and ovarian cancer cells. Once bound to the cancer cell through the folate moiety of the conjugate, curculating anti-fluorescein antibodies.may recognize and bind to the FITC moiety, resulting in antibody-dependent cellular cytotoxicity.

Physicochemical Properties

Molecular Formula	C42H34N10NA2O10S
Molecular Weight	916.824908733368
Exact Mass	895.223
CAS #	910661-33-5
Related CAS #	910661-33-5 (sodium);583037-91-6 (acid);
Appearance	Yellow to orange solid powder
Density	1.4±0.1 g/cm3
Boiling Point	591.3±50.0 °C at 760 mmHg
Flash Point	311.4±30.1 °C
Vapour Pressure	0.0±1.7 mmHg at 25°C
Index of Refraction	1.696
LogP	4.72
InChi Key	BSWYZDWNAFHWBP-ARIINYJRSA-L
InChi Code	InChI=1S/C42H36N10O10S.2Na/c43-41-51-36-35(38(57)52-41)48-23(19-47-36)18-46-21-3-1-20(2-4-21)37(56)50-30(40(60)61)11-12-33(55)44-13-14-45-42(63)49-22-5-8-26(29(15-22)39(58)59)34-27-9-6-24(53)16-31(27)62-32-17-25(54)7-10-28(32)34/h1-10,15-17,19,30,46,53H,11-14,18H2,(H,44,55)(H,50,56)(H,58,59)(H,60,61)(H2,45,49,63)(H3,43,47,51,52,57)/q2*+1/p-2/t30-/m0../s1
Chemical Name	Benzoic acid, 5-[[[[2-[[(4S)-4-[[4-[[(2-amino-1,4-dihydro-4-oxo-6-pteridinyl)methyl]amino]benzoyl]amino]-4-carboxy-1-oxobutyl]amino]ethyl]amino]thioxomethyl]amino]-2-(6-hydroxy-3-oxo-3H-xanthen-9-yl)-, sodium salt
Synonyms	EC17 EC-17 EC 17 Folate-FTIC FTIC-Folate EC17 sodium.
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The spectral binding wavelength of fluorescein, which is present in EC-17 disodium salt, is 490-530 nm. By using an ethylenediamine spacer to combine folic acid (vitamin B9) with fluorescein isothiocyanate (FITC), EC-17 disodium salt is created. Folic acid-FITC has a molecular weight of 917 kDa. FITC is a fluorescein gel that has reactive isothiocyanate groups functionalized in it. The chemical known as folic acid-FITC conjugate is fluorescent and negatively charged, and it is used to electrophorese the cell surface of FRα EC-17 disodium salt on HeLa cells. Depending on the molar concentration of Mal and concentration, the signal-to-background ratio (SBR) on HeLa cells varies from 0.97 to 7.32[1].
ln Vivo	Animals injected with EC-17 disodium salt had an average fluorescence signal of 42,234 ± 12,234 au [1]. Folate-FITC fluorescence microscopy reveals no signal in FR-α-negative or benign lesions and a high signal in all cancers expressing FR-α [2].
References	[1]. Intraoperative imaging of folate receptor alpha positive ovarian and breast cancer using the tumor specific agent EC17. Oncotarget. 2016 May 31;7(22):32144-55.

Solubility Data

Solubility (In Vitro)

DMSO : ~40 mg/mL (~43.63 mM) H2O : < 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: 1.6 mg/mL (1.75 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.6 mg/mL (1.75 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.0907 mL	5.4536 mL	10.9073 mL
	5 mM	0.2181 mL	1.0907 mL	2.1815 mL
	10 mM	0.1091 mL	0.5454 mL	1.0907 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.