(E/Z)-E64FC26 is a mixture of E-E64FC26 and Z-E64FC26. E64FC26 (E-E64FC26) is a potent pan-inhibitor of the protein disulfide isomerase (PDI) family, with IC50s of 1.9, 20.9, 25.9, 16.3, and 25.4 for PDIA1, PDIA3, PDIA4, TXNDC5, and PDIA6, respectively. μM. E64FC26 displays antimyeloma activity.

Physicochemical Properties

Molecular Formula	C19H23F3O2
Molecular Weight	340.37993645668
Exact Mass	340.165
CAS #	2285446-67-3
Related CAS #	E64FC26;2285446-62-8
PubChem CID	137796284
Appearance	Light yellow to yellow solid powder
LogP	6.5
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	7
Heavy Atom Count	24
Complexity	468
Defined Atom Stereocenter Count	0
SMILES	FC(C1=C/C(=C\CCCCCCCC)/C2C=C(C(=CC=21)O)O)(F)F
InChi Key	OWYMWLCFHDHVAH-UKTHLTGXSA-N
InChi Code	InChI=1S/C19H23F3O2/c1-2-3-4-5-6-7-8-9-13-10-16(19(20,21)22)15-12-18(24)17(23)11-14(13)15/h9-12,23-24H,2-8H2,1H3/b13-9+
Chemical Name	(1E)-1-nonylidene-3-(trifluoromethyl)indene-5,6-diol
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	PDI, Apoptosis[1][2]
References	[1]. Indene derivatives and uses thereof. US20190062298A1. [2]. Inhibitors of the protein disulfide isomerase family for the treatment of multiple myeloma. Leukemia. 2019 Apr;33(4):1011-1022.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (293.79 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (7.34 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9379 mL	14.6895 mL	29.3789 mL
	5 mM	0.5876 mL	2.9379 mL	5.8758 mL
	10 mM	0.2938 mL	1.4689 mL	2.9379 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.