Disuccinimidyl sulfoxide (DSSO) is an MS-cleavable cross-linker suitable for model peptides and proteins as well as multi-subunit protein complexes. Disuccinimidyl sulfoxide contains two symmetrical collision-induced dissociation (CID)-cleavable sites, which allow the identification of DSSO-cross-linked peptides based on their distinct fragmentation patterns.

Physicochemical Properties

Molecular Formula	C14H16N2O9S
Molecular Weight	388.35
CAS #	1351828-03-9
PubChem CID	89600516
Appearance	White to off-white solid powder
Density	1.6±0.1 g/cm3
Boiling Point	625.5±65.0 °C at 760 mmHg
Flash Point	332.1±34.3 °C
Vapour Pressure	0.0±1.8 mmHg at 25°C
Index of Refraction	1.616
LogP	-4.63
Hydrogen Bond Acceptor Count	10
Rotatable Bond Count	10
Heavy Atom Count	26
Complexity	602
Defined Atom Stereocenter Count	0
SMILES	S(CCC(ON1C(=O)CCC1=O)=O)(CCC(ON1C(=O)CCC1=O)=O)=O
Synonyms	DSSO
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Disuccinimidyl sulfoxide (DSSO) is a homobifunctional, amine-targeted, sulfoxide-containing cross-linker.
References	[1]. Development of a novel cross-linking strategy for fast and accurate identification of cross-linked peptides of protein complexes. Mol Cell Proteomics. 2011 Jan;10(1):M110.002212.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~128.75 mM; with ultrasonication) DMF : ~10 mg/mL (~25.75 mM; with heating and sonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.44 mM)(saturation unknown) in 10% DMSO 40% PEG300 5% Tween-80 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300, mix well; then add 50 μL Tween-80 to the above system, mix well; then continue to add 450 μL of normal saline to make up to 1 mL. Preparation of normal saline: Dissolve 0.9 g of sodium chloride in ddH₂O and make up to 100 mL to obtain a clear and transparent normal saline solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (6.44 mM)(saturation unknown) in 10% DMSO 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. 2 g SBE-β-CD (sulfobutyl ether β-cyclodextrin) powder is diluted to 10 mL of saline and completely dissolved until clear and transparent. Solubility in Formulation 3: ≥ 2.5 mg/mL (6.44 mM)(saturation unknown) in 10% DMSO 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL corn oil and mix well.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.5750 mL	12.8750 mL	25.7500 mL
	5 mM	0.5150 mL	2.5750 mL	5.1500 mL
	10 mM	0.2575 mL	1.2875 mL	2.5750 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.