Dioctanoylglycol (Ethylene glycol dicaprylate) is a diacylglycerol analog and an inhibitor (blocker/antagonist) of diacylglycerol kinase (DGK) (Ki 58 μM).

Physicochemical Properties

Molecular Formula	C18H34O4
Molecular Weight	314.46016
Exact Mass	314.246
CAS #	627-86-1
Related CAS #	54406-23-4
PubChem CID	3093
Appearance	Colorless to light yellow liquid
Density	0.945g/cm3
Boiling Point	387.1ºC at 760 mmHg
Flash Point	179.8ºC
Index of Refraction	1.447
LogP	4.793
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	17
Heavy Atom Count	22
Complexity	248
Defined Atom Stereocenter Count	0
SMILES	CCCCCCCC(=O)OCCOC(=O)CCCCCCC
InChi Key	HTNFLUQQANUSLR-UHFFFAOYSA-N
InChi Code	InChI=1S/C18H34O4/c1-3-5-7-9-11-13-17(19)21-15-16-22-18(20)14-12-10-8-6-4-2/h3-16H2,1-2H3
Chemical Name	2-octanoyloxyethyl octanoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Dioctanoylglycerol (dioctanoylglycerol) creates a longer-lasting diacylglycerol signal in platelets by inhibiting diacylglycerol phosphorylation (70–100% at 100 μM) [1].
References	[1]. Attenuation of sn-1,2-diacylglycerol second messengers by diacylglycerol kinase. Inhibition by diacylglycerol analogs in vitro and in human platelets. J Biol Chem. 1986 May 25;261(15):6993-7000.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~318.01 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.95 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (7.95 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.95 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.1801 mL	15.9003 mL	31.8005 mL
	5 mM	0.6360 mL	3.1801 mL	6.3601 mL
	10 mM	0.3180 mL	1.5900 mL	3.1801 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.