Dimethyl pimelimidate dihydrochloride (DMP) is a protein crosslinker that can be used in chromatin immunoprecipitation assays (ChIP) to enhance signal.

Physicochemical Properties

Molecular Formula	C9H20CL2N2O2
Molecular Weight	259.17
Exact Mass	258.09
CAS #	58537-94-3
PubChem CID	11402688
Appearance	White to off-white solid powder
Boiling Point	301.9ºC at 760mmHg
Melting Point	~122 °C (dec.)
Flash Point	136.4ºC
LogP	3.987
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	8
Heavy Atom Count	15
Complexity	151
Defined Atom Stereocenter Count	0
SMILES	Cl[H].Cl[H].O(C([H])([H])[H])/C(/C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])/C(=N/[H])/OC([H])([H])[H])=N\[H]
InChi Key	LRHXBHUTQWIZTN-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H18N2O2.2ClH/c1-12-8(10)6-4-3-5-7-9(11)13-2;;/h10-11H,3-7H2,1-2H3;2*1H
Chemical Name	dimethyl heptanediimidate;dihydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1].Chromatin immunoprecipitation (ChIP) for analysis of histone modifications and chromatin-associated proteins. Methods Mol Biol. 2009;538:409-23.

Solubility Data

Solubility (In Vitro)

H2O :~100 mg/mL (~385.85 mM; with sonication) DMSO :~100 mg/mL (385.85 mM; with sonication)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (9.65 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 400 μL PEG300 and mix well. Then add 50 μL Tween-80 to the above system and mix well. Then continue to add 450 μL of physiological saline to make up to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (9.65 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one),clear solution. For example, if 1 mL of working solution is to be prepared,you can add 100 μL of 25.0 mg/mL clear DMSO stock solution and add it to 900 μL of 20% SBE-β-CD saline solution and mix well. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8585 mL	19.2924 mL	38.5847 mL
	5 mM	0.7717 mL	3.8585 mL	7.7169 mL
	10 mM	0.3858 mL	1.9292 mL	3.8585 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.