Dihydrorhodamine 123 (DHR 123) is a fluorescent probe (λex=488 nm, λem=525 nm).

Physicochemical Properties

Molecular Formula	C21H18N2O3
Molecular Weight	346.37922
Exact Mass	346.131
CAS #	109244-58-8
PubChem CID	105032
Appearance	White to pink solid powder
Density	1.3±0.1 g/cm3
Boiling Point	526.9±50.0 °C at 760 mmHg
Flash Point	222.4±26.4 °C
Vapour Pressure	0.0±1.4 mmHg at 25°C
Index of Refraction	1.684
LogP	1.84
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	26
Complexity	488
Defined Atom Stereocenter Count	0
InChi Key	FNEZBBILNYNQGC-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H18N2O3/c1-25-21(24)15-5-3-2-4-14(15)20-16-8-6-12(22)10-18(16)26-19-11-13(23)7-9-17(19)20/h2-11,20H,22-23H2,1H3
Chemical Name	methyl 2-(3,6-diamino-9H-xanthen-9-yl)benzoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). This product is not stable in solution, please use freshly prepared working solution for optimal results.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Increased light production rate is the outcome of stimulating neutrophil NADPH oxidase with 50 nM phorbol 12-myristate 13-acetate (PMA) in the presence of 10 μM dihydrorhodamine 123 (DHR 123). After adding 50 nM PMA, the cells' fluorescence intensity increased over time in the presence of 10 μM dihydrorhodamine 123. When 50 nM PMA was added to induced HL60 cells in the presence of 10 μM dihydrorhodamine 123, the cells' fluorescence increased steadily [1].

References

[1]. Dihydrorhodamine 123: a fluorescent probe for superoxide generation? Eur.J.Biochem. 217, 973-980.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~288.70 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (7.22 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8870 mL	14.4350 mL	28.8700 mL
	5 mM	0.5774 mL	2.8870 mL	5.7740 mL
	10 mM	0.2887 mL	1.4435 mL	2.8870 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.