Dihydropalmatine is an alkaloid extracted from Berberis aristata.

Physicochemical Properties

Molecular Formula	C21H23NO4
Molecular Weight	353.4116
Exact Mass	353.163
CAS #	26067-60-7
PubChem CID	1023495
Appearance	Light yellow to yellow solid powder
Melting Point	170 °C
LogP	3.528
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	4
Heavy Atom Count	26
Complexity	524
Defined Atom Stereocenter Count	0
InChi Key	PTPHDVKWAYIFRX-UHFFFAOYSA-N
InChi Code	InChI=1S/C21H23NO4/c1-23-18-6-5-13-9-17-15-11-20(25-3)19(24-2)10-14(15)7-8-22(17)12-16(13)21(18)26-4/h5-6,9-11H,7-8,12H2,1-4H3
Chemical Name	2,3,9,10-tetramethoxy-6,8-dihydro-5H-isoquinolino[2,1-b]isoquinoline
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Rapid screening for the adulterants of Berberis aristata using direct analysis in real-time mass spectrometry and principal component analysis for discrimination. Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2015;32(6):799.

Additional Infomation

Dihydropalmatine has been reported in Thalictrum foliolosum with data available. Dihydropalmatine is a target compound for the identification of adulterants in Berberis aristata, used in combination with direct analysis in real-time mass spectrometry (DART-MS) and principal component analysis (PCA) for discrimination [1] The compound was detected in adulterated samples of Berberis aristata via DART-MS, with characteristic mass spectral peaks used to distinguish between authentic and adulterated materials [1] DART-MS analysis of Dihydropalmatine was performed under ambient conditions, enabling rapid detection without complex sample pretreatment [1]

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~70.74 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.07 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8296 mL	14.1479 mL	28.2957 mL
	5 mM	0.5659 mL	2.8296 mL	5.6591 mL
	10 mM	0.2830 mL	1.4148 mL	2.8296 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.