Dihydroevocarpine induces acute myeloid leukemia cell toxicity/cytotoxicity by inhibiting mTORC1/2 activity.

Physicochemical Properties

Molecular Formula	C23H35NO
Molecular Weight	341.53
Exact Mass	341.271
CAS #	15266-35-0
PubChem CID	5322031
Appearance	White to off-white solid powder
Density	1.0±0.1 g/cm3
Boiling Point	447.0±45.0 °C at 760 mmHg
Melting Point	68-69℃
Flash Point	149.1±18.1 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.514
LogP	8.22
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	12
Heavy Atom Count	25
Complexity	414
Defined Atom Stereocenter Count	0
InChi Key	DWHCRAGHDDLXEM-UHFFFAOYSA-N
InChi Code	InChI=1S/C23H35NO/c1-3-4-5-6-7-8-9-10-11-12-13-16-20-19-23(25)21-17-14-15-18-22(21)24(20)2/h14-15,17-19H,3-13,16H2,1-2H3
Chemical Name	1-methyl-2-tridecylquinolin-4-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Targeting of mTORC1/2 by dihydroevocarpine induces cytotoxicity in acute myeloid leukemia. J Cell Physiol. 2019;234(8):13032-13041.
Additional Infomation	Dihydroevocarpine has been reported in Tetradium ruticarpum with data available.

Solubility Data

Solubility (In Vitro)

DMSO: 16.67 mg/mL (48.81 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 1.67 mg/mL (4.89 mM) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.9280 mL	14.6400 mL	29.2800 mL
	5 mM	0.5856 mL	2.9280 mL	5.8560 mL
	10 mM	0.2928 mL	1.4640 mL	2.9280 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.