Dichotomine B, a β-carboline alkaloid, attenuates LPS/ATP-induced neuroinflammatory response in BV2 microglia.

Physicochemical Properties

Molecular Formula	C14H12N2O4
Molecular Weight	272.2561
Exact Mass	272.079
CAS #	755036-41-0
PubChem CID	11173314
Appearance	Off-white to light yellow solid powder
LogP	0.7
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	20
Complexity	378
Defined Atom Stereocenter Count	1
SMILES	O([H])[C@@]([H])(C([H])([H])O[H])C1=C2C(=C([H])C(C(=O)O[H])=N1)C1=C([H])C([H])=C([H])C([H])=C1N2[H]
InChi Key	PKSXEHHRROSXPR-NSHDSACASA-N
InChi Code	InChI=1S/C14H12N2O4/c17-6-11(18)13-12-8(5-10(16-13)14(19)20)7-3-1-2-4-9(7)15-12/h1-5,11,15,17-18H,6H2,(H,19,20)/t11-/m0/s1
Chemical Name	1-[(1R)-1,2-dihydroxyethyl]-9H-pyrido[3,4-b]indole-3-carboxylic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Dichotomine B attenuates neuroinflammatory responses via regulating TLR4/MyD88-mTOR signaling pathway in BV2 cells. Research Square. March 29th, 2022.
Additional Infomation	dichotomine B has been reported in Stellaria dichotoma with data available.

Solubility Data

Solubility (In Vitro)

DMSO : ~12.5 mg/mL (~45.91 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (4.59 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.6730 mL	18.3648 mL	36.7296 mL
	5 mM	0.7346 mL	3.6730 mL	7.3459 mL
	10 mM	0.3673 mL	1.8365 mL	3.6730 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.