Di-O-methyldemethoxycurcumin is a naturally occurring and potent curcuminoid analog with anti-inflammatory and antioxidant properties. It can be isolated from the medicinal plant Curcuma longa L and inhibits IL-6 production with an EC50 of 16.20 μg/mL.

Physicochemical Properties

Molecular Formula	C22H22O5
Molecular Weight	366.41
Exact Mass	366.147
CAS #	824951-60-2
PubChem CID	45276262
Appearance	White to yellow solid powder
LogP	3.967
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	9
Heavy Atom Count	27
Complexity	531
Defined Atom Stereocenter Count	0
SMILES	COC1=CC=C(C=C1)/C=C/C(=O)CC(=O)/C=C/C2=CC(=C(C=C2)OC)OC
InChi Key	FTMVHEMEBLBNPN-LUZURFALSA-N
InChi Code	InChI=1S/C22H22O5/c1-25-20-11-6-16(7-12-20)4-9-18(23)15-19(24)10-5-17-8-13-21(26-2)22(14-17)27-3/h4-14H,15H2,1-3H3/b9-4+,10-5+
Chemical Name	(1E,6E)-1-(3,4-dimethoxyphenyl)-7-(4-methoxyphenyl)hepta-1,6-diene-3,5-dione
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Inhibitory effects of di-O-demethylcurcumin on interleukin-1β-induced interleukin-6 production from human gingival fibroblasts. J Dent Sci. 2012 Dec;7(14): 350-358.

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~68.23 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.71 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 7.1 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7292 mL	13.6459 mL	27.2918 mL
	5 mM	0.5458 mL	2.7292 mL	5.4584 mL
	10 mM	0.2729 mL	1.3646 mL	2.7292 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.