Dehydro Palonosetron (RS 42358-197) is a specific, orally bioactive 5-HT3 receptor antagonist. Dehydro Palonosetron has no effect on the activity of 5-HT1 receptors, 5-HT2 receptors, or 5-HT4 receptors.

Physicochemical Properties

Molecular Formula	C19H22N2O
Molecular Weight	294.39
Exact Mass	296.188
CAS #	135729-56-5
PubChem CID	148210
Appearance	Typically exists as solid at room temperature
Density	1.2±0.1 g/cm3
Boiling Point	470.4±45.0 °C at 760 mmHg
Flash Point	209.5±21.1 °C
Vapour Pressure	0.0±1.2 mmHg at 25°C
Index of Refraction	1.646
LogP	2.61
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	1
Heavy Atom Count	22
Complexity	506
Defined Atom Stereocenter Count	1
SMILES	O=C1C2=CC=CC3CCC[C@@H](C2=3)CN1[C@@H]1CN2CCC1CC2
InChi Key	DIDFYSQVOPVZQB-QGZVFWFLSA-N
InChi Code	InChI=1S/C19H22N2O/c22-19-16-6-2-4-14-3-1-5-15(18(14)16)11-21(19)17-12-20-9-7-13(17)8-10-20/h2,4,6,11,13,17H,1,3,5,7-10,12H2/t17-/m1/s1
Chemical Name	2-[(3S)-1-azabicyclo[2.2.2]octan-3-yl]-5,6-dihydro-4H-benzo[de]isoquinolin-1-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	5-HT3 Receptor
ln Vitro	Dehydro Palonosetron (RS 42358-197) produced an estimated pA2 of 8.1 in in vitro functional studies, acting as a competitive antagonist against 5-HT-induced ileal contraction in guinea pigs. Neither agonistic nor antagonistic activity is observed at 5-HT1-like receptors, 5-HT2 receptors, or 5-HT4 receptors by dehydro palonosetron [1].
ln Vivo	When given intravenously, intradually, or transdermally, dehydro palonosetron (RS 42358-197) dose-dependently suppresses the 2-methyl5-HT-induced Bezold-Jarisch reflex (ID50) in rats under anesthesia. 11.6 μg/chamber, 5.7 μg/kg, iv, and 0.05 μg/kg)[1]. Dehydro Palonosetron (RS 42358-197; oral; once; 0.01 ng/kg-10 mg/kg) inhibits behaviors in mice induced by the light/dark test box's unpleasant conditions[2].
Animal Protocol	Animal/Disease Models: Male albino BKW mice (30-35 g)[2] Doses: 0.01 ng/kg, 0.1 ng/kg, 1 ng/kg, 100 ng/kg, 1 μg/kg, 100 μg/kg, 1 mg /kg, 10 mg/kg Route of Administration: Oral administration; once Experimental Results: Increased the proportion of time mice spent and the number of rearings and line crossings in the light area of the test chamber.
References	[1]. RS 42358-197, a novel and potent 5-HT3 receptor antagonist, in vitro and in vivo. J Pharmacol Exp Ther. 1993 Aug;266(2):535-43. [2]. The effect of the 5-HT3 receptor antagonist, RS-42358-197, in animal models of anxiety. Eur J Pharmacol. 1993 Mar 30;234(1):91-9.
Additional Infomation	See also: Palonosetron (annotation moved to).

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.3969 mL	16.9843 mL	33.9685 mL
	5 mM	0.6794 mL	3.3969 mL	6.7937 mL
	10 mM	0.3397 mL	1.6984 mL	3.3969 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.