DOTAP Transfection Reagent is a cationic analogue of trimethylammonium linked to two 18-carbon fatty acid tails, each with a single olefin group. 18:1 TAP is a cationic liposome-forming compound that may be utilized to transfect DNA, RNA, and other negatively charged molecules into eukaryotic cells.

Physicochemical Properties

Molecular Formula	C42H80NO4+.CH3O4S-
Molecular Weight	774.18602
Exact Mass	757.589
CAS #	144189-73-1
PubChem CID	16218742
Appearance	White to off-white solid powder
LogP	12.81
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	37
Heavy Atom Count	53
Complexity	845
Defined Atom Stereocenter Count	0
SMILES	CCCCCCCC/C=C\CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.COS(=O)(=O)[O-]
InChi Key	RSMRWWHFJMENJH-LQDDAWAPSA-M
InChi Code	InChI=1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-;
Chemical Name	2,3-bis[[(Z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;methyl sulfate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Solubility Data

Solubility (In Vitro)

DMSO : ~16.67 mg/mL (~21.53 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.67 mg/mL (2.16 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 16.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2917 mL	6.4584 mL	12.9167 mL
	5 mM	0.2583 mL	1.2917 mL	2.5833 mL
	10 mM	0.1292 mL	0.6458 mL	1.2917 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.