DNA2 inhibitor C5 is a potent, competitive, and specific inhibitor of DNA2 nuclease activity with IC50 of 20 μM. DNA2 inhibitor C5 inhibits the nuclease, DNA-dependent ATPase, helicase and DNA-binding activities of DNA2. DNA2 inhibitor C5 may be utilized in cancer-related research.

Physicochemical Properties

Molecular Formula	C10H6N2O5
Molecular Weight	234.17
Exact Mass	234.028
CAS #	35973-25-2
PubChem CID	225906
Appearance	Light yellow to yellow solid powder
Density	1.624g/cm3
Boiling Point	437.2ºC at 760mmHg
Flash Point	218.2ºC
Index of Refraction	1.674
LogP	2.07
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	1
Heavy Atom Count	17
Complexity	411
Defined Atom Stereocenter Count	0
InChi Key	BMIZBCVEHSUUNO-UHFFFAOYSA-N
InChi Code	InChI=1S/C10H6N2O5/c13-9-5-2-1-3-7(12(16)17)8(5)11-4-6(9)10(14)15/h1-4H,(H,11,13)(H,14,15)
Chemical Name	8-nitro-4-oxo-1H-quinoline-3-carboxylic acid
Synonyms	DNA2INC5; DNA2 IN C5; DNA2-IN-C5
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Dna2 inhibitors for cancer treatment. US20170226063A1.

Solubility Data

Solubility (In Vitro)

DMSO : ~31.25 mg/mL (~133.45 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (8.88 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.2704 mL	21.3520 mL	42.7040 mL
	5 mM	0.8541 mL	4.2704 mL	8.5408 mL
	10 mM	0.4270 mL	2.1352 mL	4.2704 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.