DN401 is a potent TRAP1 inhibitor with IC50 of 79 nM. DN401 has a weak inhibitory effect on Hsp90 (IC50 of 698 nM). DN401 inactivates mitochondrial TRAP1 and has potent anticancer activity.

Physicochemical Properties

Molecular Formula	C13H9BRCLN5O2
Molecular Weight	382.60
CAS #	2135749-60-7
Appearance	Off-white to light yellow solid powder
SMILES	BrC1C=C2C(=CC=1CN1C3C(=C(N=C(N)N=3)Cl)C=N1)OCO2
Synonyms	Panvotinib-401; Pan-401
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	TRAP1 79 nM (IC50); HSP90 698 nM (IC50)
ln Vitro	DN401 (5-20 µM; 24 hours) has excellent cancer-specific cytotoxicity, but has reduced cytotoxicity in normal cells[1]. DN401 (20 µM; 6 hours) inhibits the expression of Chk1 and Akt in HeLa cells[1].
ln Vivo	DN401 (30 mg/kg; intraperitoneal injection; once a day; for 14 days) reduced tumor growth and increased apoptosis in tumor tissues without causing weight loss or histological abnormalities [1].
Cell Assay	Cell Viability Assay[1] Cell Types: HeLa, SK-HEP-1, T98G, H460, and PC3 cells Tested Tested Concentrations: 5 µM, 10 µM, 15 µM, 20 µM Incubation Duration: 24 hours Experimental Results: Exhibited cytotoxic activity. Western Blot Analysis[1] Cell Types: HeLa cells Tested Tested Concentrations: 20 µM Incubation Duration: 6 hours Experimental Results: Inhibited the expression of Chk1 and Akt.
Animal Protocol	Animal/Disease Models: 6-week-old BALB/c nu/nu male mice injected with PC3 cells[1] Doses: 30 mg/kg Route of Administration: ip; once daily; for 14 days Experimental Results: Reduced tumor growth.
References	[1]. Paralog Specificity Determines Subcellular Distribution, Action Mechanism, and Anticancer Activity of TRAP1 Inhibitors. J Med Chem. 2017 Sep 14;60(17):7569-7578.

Solubility Data

Solubility (In Vitro)

DMSO : ~17.5 mg/mL (~45.74 mM; with ultrasonication (<60°C))

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6137 mL	13.0685 mL	26.1370 mL
	5 mM	0.5227 mL	2.6137 mL	5.2274 mL
	10 mM	0.2614 mL	1.3068 mL	2.6137 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.