DMNB (6-Nitroveratraldehyde) may be utilized to prepare no-carrier-added 6-[18F]fluoro-L-DOPA (6-FDOPA). DMNB (6-Nitroveratraldehyde), used in PET studies of the dopaminergic system.

Physicochemical Properties

Molecular Formula	C9H9NO5
Molecular Weight	211.17
Exact Mass	211.048
CAS #	20357-25-9
PubChem CID	88505
Appearance	Light yellow to yellow solid powder
Density	1.312 g/cm3
Boiling Point	390.8ºC at 760 mmHg
Melting Point	133.2-134.5 °C
Flash Point	194.3ºC
Vapour Pressure	2.59E-06mmHg at 25°C
Index of Refraction	1.571
LogP	1.947
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	3
Heavy Atom Count	15
Complexity	239
Defined Atom Stereocenter Count	0
SMILES	O(C([H])([H])[H])C1C([H])=C(C(C([H])=O)=C([H])C=1OC([H])([H])[H])[N+](=O)[O-]
InChi Key	YWSPWKXREVSQCA-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H9NO5/c1-14-8-3-6(5-11)7(10(12)13)4-9(8)15-2/h3-5H,1-2H3
Chemical Name	4,5-dimethoxy-2-nitrobenzaldehyde
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Zhang Lan, et al. Preparation of 6-[18F]fluoro-L-DOPA and its biodistribution in normal and unilateral PD model rats. Nuclear Science and Techniques, 13(4), 244-250. [2]. Reddy GN, et al. Quality assurance and quality control methods for animal and human applications of no-carrier-added 6-[18F]fluorodopa for PET investigations of dopaminergic systems. Appl Radiat Isot. 1993;44(7):993-998.
Additional Infomation	4,5-dimethoxy-2-nitrobenzaldehyde is a C-nitro compound and an aromatic ether.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (473.55 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (11.84 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 2.5 mg/mL (11.84 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.7355 mL	23.6776 mL	47.3552 mL
	5 mM	0.9471 mL	4.7355 mL	9.4710 mL
	10 mM	0.4736 mL	2.3678 mL	4.7355 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.