DMCM HCl is a benzodiazepine (benzodiazepine) non-selective full inverse agonist. DMCM has affinity for the human recombinant GABAA αxβ3γ2 receptor subtype, with Kis of 10 nM, 13 nM, 7.5 nM, and 2.2 nM for α1, α2, α3, and α5 receptors, respectively.

Physicochemical Properties

Molecular Formula	C17H19CLN2O4
Molecular Weight	350.796763658524
Exact Mass	350.103
CAS #	1215833-62-7
PubChem CID	45261899
Appearance	Light green to green solid powder
LogP	3.884
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	5
Rotatable Bond Count	5
Heavy Atom Count	24
Complexity	433
Defined Atom Stereocenter Count	0
InChi Key	FHCRBVQGMZUJKY-UHFFFAOYSA-N
InChi Code	InChI=1S/C17H18N2O4.ClH/c1-5-9-15-10-6-13(21-2)14(22-3)7-11(10)19-12(15)8-18-16(9)17(20)23-4;/h6-8,19H,5H2,1-4H3;1H
Chemical Name	methyl 4-ethyl-6,7-dimethoxy-9H-pyrido[3,4-b]indole-3-carboxylate;hydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo	Strong anxiogenic, proconvulsant, and convulsant effects are caused by DMCM in the body. In γ2I77 mice, DMCM (20–60 mg/kg; i.p.) has mild anxiolytic-like effects [2].
Animal Protocol	Animal/Disease Models: Male γ2I77 mice [2] Doses: 20 mg/kg and 60 mg/kg Route of Administration: intraperitoneal (ip) injection Experimental Results:Produces moderate anxiolytic-like effects.
References	[1]. An orally bioavailable, functionally selective inverse agonist at the benzodiazepine site of GABAA alpha5 receptors with cognition enhancing properties. J Med Chem. 2004 Nov 18;47(24):5829-32. [2]. Agonistic effects of the beta-carboline DMCM revealed in GABA(A) receptor gamma 2 subunit F77I point-mutated mice. Neuropharmacology. 2005 Mar;48(4):469-78.
Additional Infomation	See also: ... View More ...

Solubility Data

Solubility (In Vitro)

H2O : ~25 mg/mL (~71.27 mM) DMSO : ~10 mg/mL (~28.51 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1 mg/mL (2.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1 mg/mL (2.85 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.8506 mL	14.2531 mL	28.5063 mL
	5 mM	0.5701 mL	2.8506 mL	5.7013 mL
	10 mM	0.2851 mL	1.4253 mL	2.8506 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.