Zwittergent 3-14 (DMAPS) is a zwitterionic detergent widely used in biochemistry and molecular biology for the solubilization and purification of membrane-bound proteins and other hydrophobic biomolecules that has both hydrophilic and hydrophobic parts , giving it good detergent properties, making it suitable for stabilizing membrane proteins in aqueous solutions. In addition, DMAPS has been used in various techniques, such as electrophoresis and chromatography for the separation and analysis of biomolecules. The hydrocarbon chain provides it with good membrane penetration and solubilization capabilities, while the sulfonate and quaternary ammonium groups ensure water solubility and charge neutrality.

Physicochemical Properties

Molecular Formula	C19H41NO3S
Molecular Weight	363.60
Exact Mass	363.28
CAS #	14933-09-6
PubChem CID	84705
Appearance	White to off-white solid powder
Melting Point	250ºC
Flash Point	>110ºC
LogP	-1.05
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	16
Heavy Atom Count	24
Complexity	361
Defined Atom Stereocenter Count	0
InChi Key	BHATUINFZWUDIX-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H41NO3S/c1-4-5-6-7-8-9-10-11-12-13-14-15-17-20(2,3)18-16-19-24(21,22)23/h4-19H2,1-3H3
Chemical Name	3-[dimethyl(tetradecyl)azaniumyl]propane-1-sulfonate
Synonyms	3-(N,N-Dimethylmyristylammonio)propanesulfonate; Myristyl sulfobetaine; N-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate; Zwittergent 3-14; 3-[dimethyl(tetradecyl)azaniumyl]propane-1-sulfonate; MFCD00036910; 3-[dimethyl(tetradecyl)ammonio]propane-1-sulfonate;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Zwitterionic detergent
ln Vitro	3-(N,N-Dimethylmyristylammonio)propanesulfonate is a biochemical reagent that can be utilized in studies pertaining to life sciences as an organic substance or biological material.
References	[1]. Interchain Spacing and Screening Length Modification of PSS Backbone Chains in Zwitterion-Doped Poly(3,4-Ethylenedioxythiophene):Polystyrene Sulfonate. Journal of Surface Investigation: X-ray, Synchrotron and Neutron Techniques; Publication Date: 2020-10-08; DOI: 10.1134/s102745102007037x
Additional Infomation	Zwittergent 3-14 is an organosulfonic acid.

Solubility Data

Solubility (In Vitro)

DMSO: 8.33 mg/mL (22.91 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 400 μL of PEG300 and mix evenly; then add 50 μL of Tween-80 to the above solution and mix evenly; then add 450 μL of normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 0.83 mg/mL (2.28 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 8.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7503 mL	13.7514 mL	27.5028 mL
	5 mM	0.5501 mL	2.7503 mL	5.5006 mL
	10 mM	0.2750 mL	1.3751 mL	2.7503 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.