DMA triHCl is a fluorescent compound (λex=340 nm, λem=478 nm).

Physicochemical Properties

Molecular Formula	C27H29CLN6O2
Molecular Weight	505.011164426804
Exact Mass	576.157
CAS #	2095832-33-8
Related CAS #	DMA;188860-26-6
PubChem CID	131704506
Appearance	Light green to green solid powder
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	5
Heavy Atom Count	38
Complexity	705
Defined Atom Stereocenter Count	0
SMILES	O(C1=C(C=CC(C2=NC3=CC=C(C4=NC5=CC=C(N6CCN(C)CC6)C=C5N4)C=C3N2)=C1)OC)C.Cl
InChi Key	NJYXXGULZHPSHV-UHFFFAOYSA-N
InChi Code	InChI=1S/C27H28N6O2.3ClH/c1-32-10-12-33(13-11-32)19-6-8-21-23(16-19)31-26(29-21)17-4-7-20-22(14-17)30-27(28-20)18-5-9-24(34-2)25(15-18)35-3;;;/h4-9,14-16H,10-13H2,1-3H3,(H,28,30)(H,29,31);3*1H
Chemical Name	2-(3,4-dimethoxyphenyl)-6-[6-(4-methylpiperazin-1-yl)-1H-benzimidazol-2-yl]-1H-benzimidazole;trihydrochloride
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	The cytotoxicity of the newly synthesized bisbenzimidazole derivative DMA (6c) was assessed on human tumor cell lines (brain glioma cell line (U87), cervical cancer cell line (HeLa), and breast cancer cell line (MCF7) in comparison to Hoechst. The DMA IC50 for MCF7 is 5.3 μM. When HeLa was present, the DMA IC50 was found to be 3.4 μM [1].
References	[1]. Synthesis and biological activity of novel inhibitors of topoisomerase I: 2-aryl-substituted 2-bis-1H-benzimidazoles. Eur J Med Chem. 2011 Feb;46(2):659-69.

Solubility Data

Solubility (In Vitro)	H2O : ~15.9 mg/mL (~27.51 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 8.33 mg/mL (14.41 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.9802 mL	9.9008 mL	19.8016 mL
	5 mM	0.3960 mL	1.9802 mL	3.9603 mL
	10 mM	0.1980 mL	0.9901 mL	1.9802 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.