DL-Isocitric acid trisodium salt is an endogenously produced metabolite. DL-Isocitric acid trisodium salt is a substrate in the citric acid cycle. DL-Isocitric acid trisodium salt could be utilized as a marker for the isocitrate component in fruit products such as juices.

Physicochemical Properties

Molecular Formula	C6H5NA3O7
Molecular Weight	258.07
Exact Mass	257.972
CAS #	1637-73-6
Related CAS #	Isocitric acid;320-77-4;DL-Isocitric acid-13C4 (mixture of isomers) (trisodium hydrate);DL-Isocitric acid-13C4 trisodium salt
PubChem CID	168942
Appearance	White to light yellow solid powder
Density	1.751g/cm3
Boiling Point	329.6ºC at 760mmHg
Melting Point	86-88 °C(lit.)
Flash Point	167.4ºC
Vapour Pressure	1.3E-05mmHg at 25°C
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	2
Heavy Atom Count	16
Complexity	217
Defined Atom Stereocenter Count	0
SMILES	C(C(C(C(=O)[O-])O)C(=O)[O-])C(=O)[O-].[Na+].[Na+].[Na+]
InChi Key	HWMVXEKEEAIYGB-UHFFFAOYSA-K
InChi Code	InChI=1S/C6H8O7.3Na/c7-3(8)1-2(5(10)11)4(9)6(12)13;;;/h2,4,9H,1H2,(H,7,8)(H,10,11)(H,12,13);;;/q;3*+1/p-3
Chemical Name	trisodium;1-hydroxypropane-1,2,3-tricarboxylate
Synonyms	1637-73-6; Isocitric acid trisodium salt; DL-Isocitric acid trisodium salt hydrate; Isocitric acid, dl-; DL-Isocitric acid trisodium salt; trisodium;1-hydroxypropane-1,2,3-tricarboxylate; sodium 1-hydroxypropane-1,2,3-tricarboxylate; MFCD00149284;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Endogenous metabolite.
ln Vitro	NaHS (an H2S donor) treatment increased the activity of cultured cardiomyocytes and reduced hypertrophy in cultured cardiomyocytes at concentrations ranging from 25 to 200 µmol/L. NaHS treatment increased glucose uptake and the efficiency of glycolysis and the citric acid cycle. The key enzymes in these reactions, including lactate dehydrogenase and pyruvate kinase and succinate dehydrogenase, were activated by NaHS treatment (100 µmol/L). Some intermediates of glycolysis and the citric acid cycle, including lactic acid, cyclohexylammonium, oxaloacetic acid, succinate, L-dimalate, sodium citrate, cis-aconitic acid, ketoglutarate and DL-isocitric acid trisodium also showed anti-hypertrophic effects in cardiomyocytes [1].
Cell Assay	Introduction: Hydrogen sulfide (H2S) has been reported to inhibit myocardial hypertrophy in a cell model of cardiomyocyte hypertrophy. Our previous study also shows an H2S-induced increase in glucose metabolism in insulin-targeting cells. The present study aims to examine the hypothesis that H2S attenuates myocardial hypertrophy and promotes glucose utilization in cardiomyocytes. Methods: The cell model of cardiomyocyte hypertrophy was induced by application of phenylephrine and cardiomyocyte hypertrophy was examined using leucine incorporation assay. Protein levels were measured using Western blot analysis. The activity of related enzymes was measured with enzyme-linked immunosorbent assay (ELISA) [1].
References	[1]. Hydrogen sulfide improves glucose metabolism and prevents hypertrophy in cardiomyocytes. Nitric Oxide. 2015 Apr 30;46:114-22.
Additional Infomation	Isocitric acid, DL- is a carbonyl compound.

Solubility Data

Solubility (In Vitro)	H2O :~100 mg/mL (~387.49 mM) DMSO :< 1 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (387.49 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.8749 mL	19.3746 mL	38.7492 mL
	5 mM	0.7750 mL	3.8749 mL	7.7498 mL
	10 mM	0.3875 mL	1.9375 mL	3.8749 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.