DIPSO is a zwitterionic biological buffer with an effective pH range of 7.0 ~ 8.2. DIPSO can interfere with mouse oocyte meiosis regulation. DIPSO and is surface active at 10 mM.

Physicochemical Properties

Molecular Formula	C7H17NO6S
Molecular Weight	243.27798
Exact Mass	243.077
CAS #	68399-80-4
PubChem CID	100210
Appearance	White to off-white solid powder
Density	1.5±0.1 g/cm3
Boiling Point	449ºC
Melting Point	189-192 °C(lit.)
Index of Refraction	1.568
LogP	-2.56
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	8
Heavy Atom Count	15
Complexity	243
Defined Atom Stereocenter Count	0
SMILES	O=S(CC(O)CN(CCO)CCO)(O)=O
InChi Key	XCBLFURAFHFFJF-UHFFFAOYSA-N
InChi Code	InChI=1S/C7H17NO6S/c9-3-1-8(2-4-10)5-7(11)6-15(12,13)14/h7,9-11H,1-6H2,(H,12,13,14)
Chemical Name	3-[bis(2-hydroxyethyl)amino]-2-hydroxypropane-1-sulfonic acid
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Downs SM, Mastropolo AM. Culture conditions affect meiotic regulation in cumulus cell-enclosed mouse oocytes. Mol Reprod Dev. 1997 Apr;46(4):551-66. [2]. Copper(II) Complexation Properties and Surfactant Activity of 3-[N,N-Bis(2-hydroxyethyl)amino]-2-hydroxypropanesulfonic Acid andN-(2-Hydroxyethyl)piperazine-N′-2-hydroxypropanesulfonic Acid pH Buffers Which May Affect Trace Metal Speciation inin VitroStudies. Analytical Biochemistry, Volume 265, Issue 2, 15 December 1998, Pages 193-201.

Solubility Data

Solubility (In Vitro)	H2O : ~166.67 mg/mL (~685.10 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (205.52 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.1105 mL	20.5524 mL	41.1049 mL
	5 mM	0.8221 mL	4.1105 mL	8.2210 mL
	10 mM	0.4110 mL	2.0552 mL	4.1105 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.