DFHBI-1T is a mimic of green fluorescent protein (GFP) fluorophore used for imaging RNA in living cells. Fluorescence activated by binding to Spinach2 or Broccoli aptamers. Exhibits peak excitation maxima of 482 nm and peak fluorescence emission of 505 nm when bound to Spinach2, enabling imaging with GFP filter cubes. Exhibits higher specific fluorescence and lower background fluorescence when bound to Spinach2 compared with Spinach2-DFHBI.
Physicochemical Properties
| Molecular Formula | C13H9F5N2O2 |
| Molecular Weight | 320.214780569077 |
| Exact Mass | 320.058 |
| CAS # | 1539318-36-9 |
| Related CAS # | DFHBI-2T;1539318-40-5 |
| PubChem CID | 101889712 |
| Appearance | Light yellow to yellow solid powder |
| Density | 1.49±0.1 g/cm3(Predicted) |
| Boiling Point | 327.9±52.0 °C(Predicted) |
| Flash Point | 152.1±30.7 °C |
| Vapour Pressure | 0.0±0.7 mmHg at 25°C |
| Index of Refraction | 1.527 |
| LogP | 2.96 |
| Hydrogen Bond Donor Count | 1 |
| Hydrogen Bond Acceptor Count | 8 |
| Rotatable Bond Count | 2 |
| Heavy Atom Count | 22 |
| Complexity | 505 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | CC1=N/C(=C\C2=CC(=C(C(=C2)F)O)F)/C(=O)N1CC(F)(F)F |
| InChi Key | AWYCLBWNRONMQC-WMZJFQQLSA-N |
| InChi Code | InChI=1S/C13H9F5N2O2/c1-6-19-10(12(22)20(6)5-13(16,17)18)4-7-2-8(14)11(21)9(15)3-7/h2-4,21H,5H2,1H3/b10-4- |
| Chemical Name | (5Z)-5-[(3,5-Difluoro-4-hydroxyphenyl)methylene]-3,5-dihydro-2-methyl-3-(2,2,2-trifluoroethyl)-4H-imidazol-4-one |
| Synonyms | DFHBI-1T DFHBI1T DFHBI 1T DF-HBI-1T DF HBI 1T |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage. |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| ln Vitro | In COS7 cells expressing (CGG) 60-Spinach2, DFHBI-1T (20 μM; 10 min) increases fluorescein in comparison to DFHBI (20 μM)[1]. Broccoli-DFHBI-1T has an ex/em of 472 nm/507 nm, and spinach-2-DFHBI-1T has an ex/em of 482 nm/505 nm[2]. |
| References |
[1]. Plug-and-play fluorophores extend the spectral properties of Spinach. J Am Chem Soc. 2014 Jan 29;136(4):1198-201. [2]. Broccoli: rapid selection of an RNA mimic of green fluorescent protein by fluorescence-based selection and directed evolution. J Am Chem Soc. 2014 Nov 19;136(46):16299-308. |
Solubility Data
| Solubility (In Vitro) | DMSO : ~50 mg/mL (~156.15 mM) |
| Solubility (In Vivo) |
Solubility in Formulation 1: 5.5 mg/mL (17.18 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 55.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. (Please use freshly prepared in vivo formulations for optimal results.) |
| Preparing Stock Solutions | 1 mg | 5 mg | 10 mg | |
| 1 mM | 3.1230 mL | 15.6148 mL | 31.2295 mL | |
| 5 mM | 0.6246 mL | 3.1230 mL | 6.2459 mL | |
| 10 mM | 0.3123 mL | 1.5615 mL | 3.1230 mL |