DDR Inhibitor is a highly efficient inhibitor of the discoidin domain receptor, with IC50 of 3.3 nM for DDR2 and a 53% inhibitory activity against DDR1 at a concentration of 1.5 nM.

Physicochemical Properties

Molecular Formula	C23H20FN5O2
Molecular Weight	417.435607910156
Exact Mass	417.16
CAS #	1644069-80-6
PubChem CID	86764579
Appearance	White to off-white solid powder
LogP	3.9
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	5
Heavy Atom Count	31
Complexity	635
Defined Atom Stereocenter Count	0
SMILES	FC1=CC=CC(=C1)NC(NCC1C=CC(C)=C(C=1)NC(C1=CN=C2C=CC=CN12)=O)=O
InChi Key	ZPVUIOVIUFJGHO-UHFFFAOYSA-N
InChi Code	InChI=1S/C23H20FN5O2/c1-15-8-9-16(13-26-23(31)27-18-6-4-5-17(24)12-18)11-19(15)28-22(30)20-14-25-21-7-2-3-10-29(20)21/h2-12,14H,13H2,1H3,(H,28,30)(H2,26,27,31)
Chemical Name	N-[5-[[(3-fluorophenyl)carbamoylamino]methyl]-2-methylphenyl]imidazo[1,2-a]pyridine-3-carboxamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	DDR Inhibitor (Example 6) is a strong DDR inhibitor that exhibits 53% inhibition of DDR1 at 1.5 nM and an IC50 of 3.3 nM for DDR2 [1].
References	[1]. Imidazo-condensed bicycles as inhibitors of discoidin domain receptors (ddrs).

Solubility Data

Solubility (In Vitro)

DMSO : ~65 mg/mL (~155.71 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.17 mg/mL (5.20 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.17 mg/mL (5.20 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 21.7 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.3956 mL	11.9778 mL	23.9555 mL
	5 mM	0.4791 mL	2.3956 mL	4.7911 mL
	10 mM	0.2396 mL	1.1978 mL	2.3956 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.