DDAO is a potentially useful near-infrared (NIR) red fluorescent probe with tunable excitation wavelength (600-650nm) and long emission wavelength (λem=656nm). DDAO may be utilized to detect the activity of different enzymes like β-galactosidase, sulfatase, protein phosphatase 2A, carboxylesterase 2, human albumin and esterase.

Physicochemical Properties

Molecular Formula	C15H11NO2CL2
Molecular Weight	308.15934
Exact Mass	307.016
CAS #	118290-05-4
PubChem CID	2762655
Appearance	Purple to purplish red solid powder
Density	1.5±0.1 g/cm3
Boiling Point	449.0±45.0 °C at 760 mmHg
Flash Point	225.4±28.7 °C
Vapour Pressure	0.0±1.1 mmHg at 25°C
Index of Refraction	1.666
LogP	3.31
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	0
Heavy Atom Count	20
Complexity	550
Defined Atom Stereocenter Count	0
InChi Key	BRDJPCFGLMKJRU-UHFFFAOYSA-N
InChi Code	InChI=1S/C15H11Cl2NO2/c1-15(2)8-5-7(19)3-4-10(8)18-11-6-9(16)14(20)13(17)12(11)15/h3-6,20H,1-2H3
Chemical Name	6,8-dichloro-7-hydroxy-9,9-dimethylacridin-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. A near-infrared ratiometric/turn-on fluorescent probe for in vivo imaging of hydrogen peroxide in a murine model of acute inflammation. Anal Chim Acta. 2018 Sep 18;1024:169-176. [2]. A near-infrared ratiometric/turn-on fluorescent probe for in vivo imaging of hydrogen peroxide in a murine model of acute inflammation.Anal Chim Acta. 2018 Sep 18;1024:169-176.
Additional Infomation	1,3-dichloro-7-hydroxy-9,9-dimethyl-9H-acridin-2-one is a cyclic ketone and a member of acridines. It has a role as a fluorochrome.

Solubility Data

Solubility (In Vitro)

DMSO : ~10.42 mg/mL (~33.81 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 1.04 mg/mL (3.37 mM) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.4 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.04 mg/mL (3.37 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 10.4 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.2451 mL	16.2253 mL	32.4507 mL
	5 mM	0.6490 mL	3.2451 mL	6.4901 mL
	10 mM	0.3245 mL	1.6225 mL	3.2451 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.