DCTA monohydrate is an organic acid. DCTA refers to N,N,N',N'-tetraacetic acid, which has strong chelating ability. DCTA monohydrate works as a chelating agent and complexing reagent for metal ions. For example, DCTA monohydrate can form stable complexes with many metal ions such as calcium ions, magnesium ions, and zinc ions. DCTA, modified with ethylene glycol, is selective for calcium ions in the presence of magnesium ions.

Physicochemical Properties

Molecular Formula	C14H24N2O9
Molecular Weight	364.35
Exact Mass	364.148
CAS #	125572-95-4
PubChem CID	2723844
Appearance	White to off-white solid powder
Boiling Point	734.7ºC at 760 mmHg
Melting Point	213-216 °C(lit.)
Flash Point	398.2ºC
Vapour Pressure	9.77E-20mmHg at 25°C
Hydrogen Bond Donor Count	5
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	10
Heavy Atom Count	25
Complexity	419
Defined Atom Stereocenter Count	2
SMILES	C1CC[C@H]([C@@H](C1)N(CC(=O)O)CC(=O)O)N(CC(=O)O)CC(=O)O.O
InChi Key	VASZYFIKPKYGNC-DHTOPLTISA-N
InChi Code	InChI=1S/C14H22N2O8.H2O/c17-11(18)5-15(6-12(19)20)9-3-1-2-4-10(9)16(7-13(21)22)8-14(23)24;/h9-10H,1-8H2,(H,17,18)(H,19,20)(H,21,22)(H,23,24);1H2/t9-,10-;/m1./s1
Chemical Name	2-[[(1R,2R)-2-[bis(carboxymethyl)amino]cyclohexyl]-(carboxymethyl)amino]acetic acid;hydrate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Measuring of water transport selectively along the plant root plasmodesmata using gradient nuclear magnetic resonance with paramagnetic doping. Plant Physiol Biochem. 2023 Jan;194:263-270. [2]. The Selective Chelatometry of Calcium in the Presence of Magnesium with Ethylene Glycol-bis (β-aminoethyl ether)-N, N, N′, N′-tetraacetic Acid. Bulletin of the Chemical Society of Japan, 1963, 36(3): 320-324.

Solubility Data

Solubility (In Vitro)

DMSO: 25 mg/mL (68.62 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.86 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7446 mL	13.7231 mL	27.4461 mL
	5 mM	0.5489 mL	2.7446 mL	5.4892 mL
	10 mM	0.2745 mL	1.3723 mL	2.7446 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.