DCEBIO, an analogue of 1-EBIO, is a highly potent activator of Cl－ secretion in T84 colonic cells. DCEBIO stimulates Cl－ secretion via the activation of hIK1 K＋ channels and the activation of an apical membrane Cl－ conductance.

Physicochemical Properties

Molecular Formula	C9H8N2OCL2
Molecular Weight	231.07862
Exact Mass	230.001
CAS #	60563-36-2
PubChem CID	656765
Appearance	White to light brown solid powder
Density	1.413g/cm3
Melting Point	209.5-210.5 °C
Index of Refraction	1.592
LogP	2.656
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	1
Rotatable Bond Count	1
Heavy Atom Count	14
Complexity	249
Defined Atom Stereocenter Count	0
InChi Key	LKHRMULASXZCLG-UHFFFAOYSA-N
InChi Code	InChI=1S/C9H8Cl2N2O/c1-2-13-8-4-6(11)5(10)3-7(8)12-9(13)14/h3-4H,2H2,1H3,(H,12,14)
Chemical Name	5,6-dichloro-3-ethyl-1H-benzimidazol-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Hamilton KL,et al. DCEBIO stimulates Cl- secretion in the mouse jejunum. Am J Physiol Cell Physiol. 2006 Jan;290(1):C152-64. [2]. Singh\nS, et al. Benzimidazolone activators of chloride secretion: potential\ntherapeutics for cystic fibrosis and chronic obstructive pulmonary\ndisease. J Pharmacol Exp Ther. 2001 Feb;296(2):600-11.
Additional Infomation	DCEBIO is a dichlorobenzene.

Solubility Data

Solubility (In Vitro)

DMSO : ~50 mg/mL (~216.38 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.82 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (10.82 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3275 mL	21.6375 mL	43.2751 mL
	5 mM	0.8655 mL	4.3275 mL	8.6550 mL
	10 mM	0.4328 mL	2.1638 mL	4.3275 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.