DC-TEADin02 is a potent inhibitor of TEAD autopalmitoylation. DC-TEADin02 inhibits TEAD autopalmitoylation with IC50 of 197 nM. DC-TEADin02 may be utilized to study development, regeneration, and tissue homeostasis.

Physicochemical Properties

Molecular Formula	C19H17NO2S
Molecular Weight	323.41
Exact Mass	323.097
CAS #	2380228-45-3
PubChem CID	155527561
Appearance	White to off-white solid powder
LogP	4.5
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	5
Heavy Atom Count	23
Complexity	488
Defined Atom Stereocenter Count	0
SMILES	C(S(NC1=CC=C(CC2=CC=C3C(=C2)C=CC=C3)C=C1)(=O)=O)=C
InChi Key	IUZGUIPYVGPQFT-UHFFFAOYSA-N
InChi Code	InChI=1S/C19H17NO2S/c1-2-23(21,22)20-19-11-8-15(9-12-19)13-16-7-10-17-5-3-4-6-18(17)14-16/h2-12,14,20H,1,13H2
Chemical Name	N-[4-(naphthalen-2-ylmethyl)phenyl]ethenesulfonamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	DC-TEADin02 inhibits TEAD autopalmitoylation with an IC50 value of 197 nM[1]. The downregulation of downstream gene expression related to YAP is caused by the inhibition of TEAD transcriptional activity by DC-TEADin02 (0-25 μM) [1].
References	[1]. Discovery and biological evaluation of vinylsulfonamide derivatives as highly potent, covalent TEAD autopalmitoylation inhibitors. Eur J Med Chem. 2019 Dec 15;184:111767.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (309.21 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (7.73 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.5 mg/mL (7.73 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 2.5 mg/mL (7.73 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0921 mL	15.4603 mL	30.9205 mL
	5 mM	0.6184 mL	3.0921 mL	6.1841 mL
	10 mM	0.3092 mL	1.5460 mL	3.0921 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.