DAB (tetra HCl hydrate) is extensively used as a chromogenic substrate in immunohistochemistry and enzymatic histochemistry to visualize the antigen or enzyme of interest. When oxidized by hydrogen peroxide, DAB (tetra HCl hydrate) will produce a brown precipitate at the location of the antigen or enzyme, which is easy to detect and observe under a microscope. In addition, DAB (tetra HCl hydrate) also may be utilized in other research areas, such as the development of biosensors and the study of cellular oxidative stress.

Physicochemical Properties

Molecular Formula	C12H18CL4N4
Molecular Weight	360.11
Exact Mass	376.039
CAS #	868272-85-9
Related CAS #	DAB tetrahydrochloride;7411-49-6;DAB;91-95-2
PubChem CID	23892
Appearance	Solid
Melting Point	300°C
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	1
Heavy Atom Count	20
Complexity	209
Defined Atom Stereocenter Count	0
SMILES	Cl.O.NC1C(N)=CC(C2C=C(N)C(N)=CC=2)=CC=1
InChi Key	KJDSORYAHBAGPP-UHFFFAOYSA-N
InChi Code	InChI=1S/C12H14N4.4ClH/c13-9-3-1-7(5-11(9)15)8-2-4-10(14)12(16)6-8;;;;/h1-6H,13-16H2;4*1H
Chemical Name	4-(3,4-diaminophenyl)benzene-1,2-diamine;tetrahydrochloride
Synonyms	3,3'-Diaminobenzidine tetrahydrochloride hydrate; 868272-85-9; [1,1'-Biphenyl]-3,3',4,4'-tetraamine tetrahydrochloride hydrate; 4-(3,4-diaminophenyl)benzene-1,2-diamine;hydrate;tetrahydrochloride; MFCD08273058; DAB; C12H20Cl4N4O; C12H14N4.4HCl;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Biochemical Assay Reagent
ln Vitro	A biochemical reagent called DAB 4HCl Hydrate can be utilized in life science research as an organic substance or biological material.
Toxicity/Toxicokinetics	Non-Human Toxicity Values LD50 Mouse ip 330 mg/kg
Additional Infomation	3,3'-diaminobenzidine is a member of the class of biphenyls that is benzidine in which one of the hydrogens ortho to each of the amino groups has been replaced by an amino group. It has a role as a histological dye. It is a member of biphenyls and a substituted aniline. A chemically and thermodynamically stable derivative of BENZIDINE.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (277.69 mM) H2O: 50 mg/mL (138.85 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.7769 mL	13.8846 mL	27.7693 mL
	5 mM	0.5554 mL	2.7769 mL	5.5539 mL
	10 mM	0.2777 mL	1.3885 mL	2.7769 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.