D-(+)-Trehalose dihydrate is extracted from Saccharomyces cerevisiae and can be used as a food ingredient and active molecular excipient.

Physicochemical Properties

Molecular Formula	C12H26O13
Molecular Weight	378.3270
Exact Mass	378.137
CAS #	6138-23-4
Related CAS #	D-(+)-Trehalose;99-20-7
PubChem CID	181978
Appearance	White to off-white solid powder
Density	1.76 g/cm3
Boiling Point	675.4ºC at 760 mmHg
Melting Point	97-99 °C(lit.)
Flash Point	362.3ºC
Index of Refraction	181 ° (C=7, H2O)
Hydrogen Bond Donor Count	10
Hydrogen Bond Acceptor Count	13
Rotatable Bond Count	4
Heavy Atom Count	25
Complexity	348
Defined Atom Stereocenter Count	10
SMILES	C([C@@H]1[C@H]([C@@H]([C@H]([C@H](O1)O[C@@H]2[C@@H]([C@H]([C@@H]([C@H](O2)CO)O)O)O)O)O)O)O.O.O
InChi Key	DPVHGFAJLZWDOC-PVXXTIHASA-N
InChi Code	InChI=1S/C12H22O11.2H2O/c13-1-3-5(15)7(17)9(19)11(21-3)23-12-10(20)8(18)6(16)4(2-14)22-12;;/h3-20H,1-2H2;2*1H2/t3-,4-,5-,6-,7+,8+,9-,10-,11-,12-;;/m1../s1
Chemical Name	(2R,3S,4S,5R,6R)-2-(hydroxymethyl)-6-[(2R,3R,4S,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]oxyoxane-3,4,5-triol;dihydrate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Trehalose dihydrate is a harmless natural disaccharide that is used as a food additive and medicinal excipient [1]..
References	[1]. Amorphous trehalose dihydrate by cryogenic milling. Carbohydr Res. 2011 Jun 1;346(8):1061-4.

Solubility Data

Solubility (In Vitro)	H2O : ~150 mg/mL (~396.48 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 50 mg/mL (132.16 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6432 mL	13.2160 mL	26.4320 mL
	5 mM	0.5286 mL	2.6432 mL	5.2864 mL
	10 mM	0.2643 mL	1.3216 mL	2.6432 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.