D-Biotinol is the nutritional component of Lactobacillus arabiensis, Lactobacillus casei or Saccharomyces cerevisiae. D-Biotinol can replace D-biotin to restore egg white-induced biotin deficiency in rats. D-Biotinol is orally bioactive and can be converted into biotin by rats.

Physicochemical Properties

Molecular Formula	C10H18N2O2S
Molecular Weight	230.33
Exact Mass	230.109
CAS #	53906-36-8
PubChem CID	10082587
Appearance	White to off-white solid powder
LogP	1.362
Hydrogen Bond Donor Count	3
Hydrogen Bond Acceptor Count	3
Rotatable Bond Count	5
Heavy Atom Count	15
Complexity	238
Defined Atom Stereocenter Count	3
SMILES	C1[C@H]2[C@@H]([C@@H](S1)CCCCCO)NC(=O)N2
InChi Key	RGIKRHKHRAAZIO-CIUDSAMLSA-N
InChi Code	InChI=1S/C10H18N2O2S/c13-5-3-1-2-4-8-9-7(6-15-8)11-10(14)12-9/h7-9,13H,1-6H2,(H2,11,12,14)/t7-,8-,9-/m0/s1
Chemical Name	(3aS,4S,6aR)-4-(5-hydroxypentyl)-1,3,3a,4,6,6a-hexahydrothieno[3,4-d]imidazol-2-one
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: (1). This product requires protection from light (avoid light exposure) during transportation and storage.(2). Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Utilization of d-Biotinol by Microorganisms, the Rat and Human. Proceedings of the Society for Experimental Biology and Medicine, 1951, 78(2): 381-383.

Solubility Data

Solubility (In Vitro)

DMSO: 100 mg/mL (434.16 mM) Methanol: 25 mg/mL (108.54 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (10.85 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	4.3416 mL	21.7080 mL	43.4160 mL
	5 mM	0.8683 mL	4.3416 mL	8.6832 mL
	10 mM	0.4342 mL	2.1708 mL	4.3416 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.