Cytidine 5′-triphosphate (Cytidine triphosphate; 5'-CTP) is a nucleoside triphosphate that serves as a building block in the biosynthesis of nucleotides and nucleic acids, and lipids. Cytosine triphosphate synthase can catalyze UTP to generate 5'-CTP. Cytidine 5′-triphosphate is an essential biomolecule in the de novo pyrimidine biosynthetic pathway of T. gondii.

Physicochemical Properties

Molecular Formula	C9H16N3O14P3
Molecular Weight	483.16
Exact Mass	482.984
CAS #	65-47-4
Related CAS #	Cytidine-5'-triphosphate disodium;36051-68-0;Cytidine-5′-triphosphate-d14 disodium
PubChem CID	6176
Appearance	White to off-white solid powder
Density	2.5±0.1 g/cm3
Boiling Point	849.2±75.0 °C at 760 mmHg
Melting Point	215 - 218 °C
Flash Point	467.4±37.1 °C
Vapour Pressure	0.0±0.6 mmHg at 25°C
Index of Refraction	1.801
LogP	-5.52
Hydrogen Bond Donor Count	7
Hydrogen Bond Acceptor Count	14
Rotatable Bond Count	8
Heavy Atom Count	29
Complexity	855
Defined Atom Stereocenter Count	4
SMILES	C1=CN(C(=O)N=C1N)[C@H]2[C@@H]([C@@H]([C@H](O2)COP(=O)(O)OP(=O)(O)OP(=O)(O)O)O)O
InChi Key	PCDQPRRSZKQHHS-XVFCMESISA-N
InChi Code	InChI=1S/C9H16N3O14P3/c10-5-1-2-12(9(15)11-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H2,10,11,15)(H2,16,17,18)/t4-,6-,7-,8-/m1/s1
Chemical Name	[[(2R,3S,4R,5R)-5-(4-amino-2-oxopyrimidin-1-yl)-3,4-dihydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl] phosphono hydrogen phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	Human Endogenous Metabolite
References	[1]. A CTP Synthase Undergoing Stage-Specific Spatial Expression Is Essential for the Survival of the Intracellular Parasite Toxoplasma gondii. Front Cell Infect Microbiol. 2018 Mar 22;8:83.
Additional Infomation	CTP is a pyrimidine ribonucleoside 5'-triphosphate and a cytidine 5'-phosphate. It has a role as an Escherichia coli metabolite and a mouse metabolite. It is a conjugate acid of a CTP(4-) and a CTP(3-). Cytidine triphosphate is a metabolite found in or produced by Escherichia coli (strain K12, MG1655). Cytidine-5'-triphosphate has been reported in Homo sapiens, Apis cerana, and Caenorhabditis elegans with data available. Cytidine Triphosphate is a cytosine derived nucleotide comprised of three phosphate groups esterified to the deoxyribose sugar moiety. Nucleotide metabolism is upregulated in cancer cells; therefore, this nucleotide could be used as a biomarker to detect the presence of cancer cells. Cytidine triphosphate is a metabolite found in or produced by Saccharomyces cerevisiae. Cytidine 5'-(tetrahydrogen triphosphate). A cytosine nucleotide containing three phosphate groups esterified to the sugar moiety.

Solubility Data

Solubility (In Vitro)	H2O : 83.33 mg/mL (172.47 mM) DMSO : < 1 mg/mL
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (206.97 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.0697 mL	10.3485 mL	20.6971 mL
	5 mM	0.4139 mL	2.0697 mL	4.1394 mL
	10 mM	0.2070 mL	1.0349 mL	2.0697 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.