Cytarabine 5′-monophosphate is a metabolite of the nucleoside analog cytarabine catalyzed by deoxycytidine kinase. Cytarabine 5′-monophosphate is incorporated into DNA by DNA polymerase α, which can reduce the rate of DNA synthesis. Cytarabine 5′-monophosphate inhibits the replication of nuclear DNA and mitochondrial DNA in Saccharomyces cerevisiae at a concentration of 15 mM. In addition, Cytarabine 5′-monophosphate (3.5-75.1 mg/kg) can improve the survival rate of leukemia mice (L1210 mice). Cytarabine 5′-monophosphate can be used in cancer research.

Physicochemical Properties

Molecular Formula	C9H14N3O8P
Molecular Weight	323.20
CAS #	7075-11-8
Related CAS #	Cytarabine-13C3 5'-monophosphate
SMILES	O=C1N=C(N)C=CN1[C@H]2[C@H]([C@@H]([C@@H](COP(O)(O)=O)O2)O)O
Synonyms	Cytarabine-5'-monophosphate
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Mammalian deoxynucleoside kinase. I. Deoxycytidine kinase: purification, properties, and kinetic studies with cytosine arabinoside. J Biol Chem. 1968 Aug 25;243(16):4298-304. [2]. Development and application of a rapid and sensitive liquid chromatography-mass spectrometry method for simultaneous analysis of cytarabine, cytarabine monophosphate, cytarabine diphosphate and cytarabine triphosphate in the cytosol and nucleus. J Pharm Biomed Anal. 2022 Mar 20;211:114582. [3]. Incorporation of cytosine arabinoside monophosphate into DNA at internucleotide linkages by human DNA polymerase alpha. J Biol Chem. 1992 Nov 15;267(32):23043-51. [4]. Inhibition of DNA replication in Saccharomyces cerevisiae by araCMP. Curr Genet. 1986;10(8):579-85. [5]. Antitumor effect and mode of action of 1-beta-D-arabinofuranosylcytosine 5'-phosphate in leukemia L1210. Cancer Res. 1968 Apr;28(4):802-3.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.0941 mL	15.4703 mL	30.9406 mL
	5 mM	0.6188 mL	3.0941 mL	6.1881 mL
	10 mM	0.3094 mL	1.5470 mL	3.0941 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.