Cypate is a near-infrared fluorescent dye that belongs to the photosensitizer family. Cypate has high photostability and optical properties and is widely utilized in near-infrared optical imaging, optical development, tumor labeling, drug delivery, etc. In addition, Cypate is also used as a molecular probe and combined with targeting molecules (such as CBT or small interfering RNA) to achieve efficient detection and imaging of specific cells or tissues.

Physicochemical Properties

Molecular Formula	C41H41CLN2O4
Molecular Weight	661.23
Related CAS #	Cypate;95837-47-1
Appearance	Green to dark green solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

According to the study, Cypate coupled upconversion nanoparticles (UCNP-cy) loaded with anti-heat shock protein 70 small interfering RNA genes (UCNP-cy-siRNA) can cause targeted cellular damage and have anti-tumor properties [1]. In order to track, label, and photograph particular biomolecules or cells, Cypate can also be connected to CBT (Cysteine-containing Peptide Backbone Tag). For instance, cathepsin B (CTSB) activity can be specifically seen and tracked in real time in tumors and cells that overexpress CTSB using Cypate-CBT, a near-infrared photoacoustic (PA) probe. Cypate-CBT increases the strength and duration of the PA signal at the tumor site by entering CTSB-overexpressing cells, where it is then subjected to glutathione reduction and CTSB cleavage to produce cypate nanoparticles, or Cypate-CBT-NPs. For the clinical diagnosis of early cancer, cipate-CBT has the potential to be a useful PA imaging agent [2].

References

[1]. Cypate-conjugated porous upconversion nanocomposites for programmed delivery of heat shock protein 70 small interfering RNA for gene silencing and photothermal ablation. Advanced Functional Materials, 2016, 26(20): 3480-3489. [2]. Cathespin B-Initiated Cypate Nanoparticle Formation for Tumor Photoacoustic Imaging. Angew Chem Int Ed Engl. 2022 Jan 26;61(5):e202114766.

Solubility Data

Solubility (In Vitro)

DMSO :~12.5 mg/mL (~18.90 mM) H2O :< 0.1 mg/mL

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.25 mg/mL (1.89 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: 1.25 mg/mL (1.89 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with ultrasonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 12.5 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5123 mL	7.5617 mL	15.1233 mL
	5 mM	0.3025 mL	1.5123 mL	3.0247 mL
	10 mM	0.1512 mL	0.7562 mL	1.5123 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.