Creatine-d3 (hydrate) is the deuterium labelled form ofCreatine hydrate. Creatine hydrate plays a crucial role in energy metabolism in muscle and brain cells.

Physicochemical Properties

Molecular Formula	C4H9N3O2.H2O
Molecular Weight	149.14844
Exact Mass	152.098
CAS #	284664-86-4
Related CAS #	Creatine;57-00-1
PubChem CID	16213871
Appearance	White to off-white solid powder
Melting Point	292ºC (dec.)(lit.)
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	3
Heavy Atom Count	10
Complexity	134
Defined Atom Stereocenter Count	0
SMILES	[2H]C([2H])([2H])N(CC(=O)O)C(=N)N.O
InChi Key	MEJYXFHCRXAUIL-NIIDSAIPSA-N
InChi Code	InChI=1S/C4H9N3O2.H2O/c1-7(4(5)6)2-3(8)9;/h2H2,1H3,(H3,5,6)(H,8,9);1H2/i1D3;
Chemical Name	2-[carbamimidoyl(trideuteriomethyl)amino]acetic acid;hydrate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Beyond sports: Efficacy and safety of creatine supplementation in pathological or paraphysiological conditions of brain and muscle. Med Res Rev. 2019;39(6):2427-2459.

Solubility Data

Solubility (In Vitro)	H2O : ~25 mg/mL (~164.29 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 6.67 mg/mL (43.83 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	6.7047 mL	33.5233 mL	67.0466 mL
	5 mM	1.3409 mL	6.7047 mL	13.4093 mL
	10 mM	0.6705 mL	3.3523 mL	6.7047 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.