Physicochemical Properties
| Molecular Formula | C15H14O3 |
| Exact Mass | 242.094 |
| CAS # | 82344-82-9 |
| PubChem CID | 11390848 |
| Appearance | Typically exists as solid at room temperature |
| LogP | 2.872 |
| Hydrogen Bond Donor Count | 2 |
| Hydrogen Bond Acceptor Count | 3 |
| Rotatable Bond Count | 1 |
| Heavy Atom Count | 18 |
| Complexity | 294 |
| Defined Atom Stereocenter Count | 0 |
| SMILES | OC1C=C2CCC3C(C2=CC=1)=C(OC)C=C(O)C=3 |
| InChi Key | OPPGAHUCKDKQJR-UHFFFAOYSA-N |
| InChi Code | InChI=1S/C15H14O3/c1-18-14-8-12(17)7-10-3-2-9-6-11(16)4-5-13(9)15(10)14/h4-8,16-17H,2-3H2,1H3 |
| Chemical Name | 4-methoxy-9,10-dihydrophenanthrene-2,7-diol |
| HS Tariff Code | 2934.99.9001 |
| Storage |
Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month |
| Shipping Condition | Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs) |
Biological Activity
| Targets | Akt NF-κB IL-1β IL-6 |
| ln Vitro | The phosphorylation levels of NF-κB p65 and p105/50 are considerably reduced by coelonin (2.5 μg/mL) [1]. Dose-dependently, coelonin (0–5 μg/mL, 1.5 h) lowers the rise in PTEN, AKT, and IκBa phosphorylation produced by LPS [1]. By lowering the synthesis of inflammatory factors like interleukin 6 (IL-6) and tumor necrosis factor-alpha (TNF-alpha), coelonin (10 and 20 μg/ml) lowers inflammation caused by particulate matter 2.5 (PM2.5). [2]. Coelonin inhibits the expression of IL-1β, IL-6, and TNF-α without the need for PTEN, but it requires PTEN to prevent the degradation of p27Kip1, which results in cell cycle arrest in the G1 phase[1]. |
| Cell Assay |
Western Blot Analysis[1] Cell Types: RAW264.7 cells Tested Concentrations: 0, 1, 2.5, and 5 μg/mL Incubation Duration: 1.5 h Experimental Results: Dose dependently decreased the increase of p65 accumulation in the nucleus induced by LPS. Dose dependently reversed LPS-induced iNOS and COX2 expression. LPS (200 ng/mL) Dramatically increased the phosphorylation of PTEN, AKT and inhibitor of NF-κB (IκBa), which was dose-dependently decreased by coelonin pre-treatment. |
| References |
[1]. Coelonin, an Anti-Inflammation Active Component of Bletilla striata and Its Potential Mechanism. Int J Mol Sci. 2019 Sep 8;20(18):4422. [2]. Inhibition of inflammation-induced injury and cell migration by coelonin and militarine in PM2.5-exposed human lung alveolar epithelial A549 cells. Eur J Pharmacol. 2021 Apr 5;896:173931. |
| Additional Infomation | 4-Methoxy-9,10-dihydrophenanthrene-2,7-diol has been reported in Coelogyne elata, Calanthe arisanensis, and other organisms with data available. |
Solubility Data
| Solubility (In Vitro) | May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples |
| Solubility (In Vivo) |
Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently. (Please use freshly prepared in vivo formulations for optimal results.) |