Cl-Necrostatin-1 is a RIPK1 inhibitor. Cl-Necrostatin-1 also inhibits TNF-α-induced necroptosis in Jurkat cells lacking Fas-associated death domain protein (FADD) (EC50 of 180 nM), a change that prevents death domain receptor signaling from activating apoptotic enzymes. Cl-Necrostatin-1 also reduces infarct size in a mouse model of cerebral artery occlusion (MCAO). Cl-Necrostatin-1 can be used in cardiovascular and cerebrovascular disease research.

Physicochemical Properties

Molecular Formula	C13H12CLN3OS
Molecular Weight	293.77
CAS #	862377-51-3
SMILES	ClC1C2=C(C(CC3NC(=S)N(C)C3=O)=CN2)C=CC=1
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References

[1]. Identification of RIP1 kinase as a specific cellular target of necrostatins. Nat Chem Biol. 2008 May;4(5):313-21. [2]. Structure-activity relationship study of novel necroptosis inhibitors. Bioorg Med Chem Lett. 2005 Nov 15;15(22):5039-44. [3]. Chemical inhibitor of nonapoptotic cell death with therapeutic potential for ischemic brain injury. Nat Chem Biol. 2005 Jul;1(2):112-9.

Solubility Data

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	3.4040 mL	17.0201 mL	34.0402 mL
	5 mM	0.6808 mL	3.4040 mL	6.8080 mL
	10 mM	0.3404 mL	1.7020 mL	3.4040 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.