Cjoc42 is a compound that binds to gankyrin. Cjoc42 inhibits gankyrin activity in a dose-dependent fashion. Cjoc42 prevents the decrease in p53 protein levels normally associated with high amounts of gankyrin. Cjoc42 restores p53-dependent transcription and sensitivity to DNA damage.

Physicochemical Properties

Molecular Formula	C20H21N3O5S
Molecular Weight	415.46
Exact Mass	415.12
CAS #	2171519-89-2
Appearance	Off-white to light yellow solid powder
LogP	3.2
SMILES	C(OC)(=O)C1=CC=C(N2C=C(CCCOS(C3=CC=C(C)C=C3)(=O)=O)N=N2)C=C1
InChi Key	VYUDJPGSULLWAS-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H21N3O5S/c1-15-5-11-19(12-6-15)29(25,26)28-13-3-4-17-14-23(22-21-17)18-9-7-16(8-10-18)20(24)27-2/h5-12,14H,3-4,13H2,1-2H3
Chemical Name	methyl 4-[4-[3-(4-methylphenyl)sulfonyloxypropyl]triazol-1-yl]benzoate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	When compared to mock-transfected cells, cjoc42 (0.5, 1 and 5 μM; 48 hours) dose-dependently restores p53 levels and prevents gankyrin-induced p53 level drop in cells [1]. In terms of cell viability, cjoc42 alone (0.1, 1 and 10 μM; 24 hours) did not exhibit any dose-dependent alterations [1].
Cell Assay	Western Blot Analysis[1] Cell Types: U2OS Tested Concentrations: 0.5, 1 and 5 μM Incubation Duration: 48 hrs (hours) Experimental Results: Dose-dependent restoration of p53 levels (compared to mock-transfected cells) and inhibition of gankyrin-induced decrease in p53 levels in cells .
References	[1]. Discovery of a small-molecule binder of the oncoprotein gankyrin that modulates gankyrin activity in the cell. Sci Rep. 2016;6:23732.

Solubility Data

Solubility (In Vitro)

DMSO : ~100 mg/mL (~240.70 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (6.02 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.4070 mL	12.0349 mL	24.0697 mL
	5 mM	0.4814 mL	2.4070 mL	4.8139 mL
	10 mM	0.2407 mL	1.2035 mL	2.4070 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.