Chlorhexidine acetate hydrate is an antimicrobial and preservative. Chlorhexidine acetate hydrate may be utilized to cleanse the skin after trauma, before surgery, or before injections.

Physicochemical Properties

Molecular Formula	C22H30CL2N10.2C2H4O2.XH2O
Molecular Weight	625.55052
Exact Mass	624.245
CAS #	206986-79-0
Related CAS #	Chlorhexidine (digluconate);18472-51-0;Chlorhexidine;55-56-1;Chlorhexidine diacetate;56-95-1
PubChem CID	9562059
Appearance	Typically exists as solid at room temperature
Boiling Point	699.3ºC at 760 mmHg
Melting Point	153-157ºC
Flash Point	376.7ºC
LogP	6.465
Hydrogen Bond Donor Count	8
Hydrogen Bond Acceptor Count	6
Rotatable Bond Count	13
Heavy Atom Count	42
Complexity	680
Defined Atom Stereocenter Count	0
SMILES	CC(=O)O.CC(=O)O.N/C(/N=C(/NC1C=CC(Cl)=CC=1)\N)=N\CCCCCC/N=C(/N=C(/NC1C=CC(Cl)=CC=1)\N)\N
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vivo	Chlorhexidine acetate hydrate acts as a preservative and antimicrobial. The skin can be cleaned with chlorhexidine acetate hydrate following trauma, prior to surgery, or before injections.
Animal Protocol	Animal/Disease Models: One hundred and eighty male Imprinting Control Region (ICR) mice at 6 to8-wk-old (body weight, 25 ± 3 g) were randomized to six groups[1]. Doses: Chlorhexidine gluconate 0.05%, and Chlorhexidine gluconate 0.025%. Route of Administration: One-time intraperitoneal (ip)injection. Experimental Results: A total of 48 mice (26.7%) developed postoperative intra-abdominal abscesses. Mice that had Chlorhexidine gluconate 0.05% lavage had Dramatically lower incidence of postoperative intra-abdominal abscesses compared with that of group D mice that had saline lavage only (P = 0.0113). Microscopic peritoneal fibrosis occurred Dramatically more among group E mice that had Chlorhexidine gluconate 0.05% lavage compared with group D mice that had saline lavage only (P = < 0.005).
References	[1]. Wael E Shams, et al. Peritoneal Lavage Using Chlorhexidine Gluconate at the End of Colon Surgery Reduces Postoperative Intra-Abdominal Infection in Mice. J Surg Res. 2015 May 1;195(1):121-7.
Additional Infomation	Chlorhexidine acetate is the acetate salt of chlorhexidine. It is a disinfectant used for bacterial control in hospital, agricultural and domestic environments. It has a role as an antibacterial agent, an antiinfective agent, an antifungal agent and an antifouling biocide. It contains a chlorhexidine. A disinfectant and topical anti-infective agent used also as mouthwash to prevent oral plaque.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.5986 mL	7.9930 mL	15.9859 mL
	5 mM	0.3197 mL	1.5986 mL	3.1972 mL
	10 mM	0.1599 mL	0.7993 mL	1.5986 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.