Ceapin-A7 is a selective blocker of endoplasmic reticulum stress ATF6α signaling with IC50 of 0.59 μM. Ceapin-A7 may be utilized to explore the mechanism of ATF6α promoting cell activation and its role in pathological environments.

Physicochemical Properties

Molecular Formula	C20H12F6N4O3
Molecular Weight	470.3247
Exact Mass	470.081
CAS #	2323027-38-7
PubChem CID	154730128
Appearance	Off-white to light yellow solid powder
LogP	4
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	11
Rotatable Bond Count	5
Heavy Atom Count	33
Complexity	694
Defined Atom Stereocenter Count	0
SMILES	FC(C1C=C(C(F)(F)F)C=CC=1CN1C=C(C=N1)NC(C1C=C(C2=CC=CO2)ON=1)=O)(F)F
InChi Key	UJTDYOXTXGBHEG-UHFFFAOYSA-N
InChi Code	InChI=1S/C20H12F6N4O3/c21-19(22,23)12-4-3-11(14(6-12)20(24,25)26)9-30-10-13(8-27-30)28-18(31)15-7-17(33-29-15)16-2-1-5-32-16/h1-8,10H,9H2,(H,28,31)
Chemical Name	N-[1-[[2,4-bis(trifluoromethyl)phenyl]methyl]pyrazol-4-yl]-5-(furan-2-yl)-1,2-oxazole-3-carboxamide
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	0.59 μM (ATF6α)[1]
ln Vitro	Ceapin-A7 increases a cell's sensitivity to ER stress [1]. ATF6α but not ATF6β is selectively inhibited by ceapin (0.6–18.9 μM; 4.5 hours)[1].
References	[1]. Ceapins are a new class of unfolded protein response inhibitors, selectively targeting the ATF6α branch. eLife. 2016; 5: e11878.

Solubility Data

Solubility (In Vitro)

DMSO : 100 mg/mL (212.62 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.5 mg/mL (5.32 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1262 mL	10.6311 mL	21.2621 mL
	5 mM	0.4252 mL	2.1262 mL	4.2524 mL
	10 mM	0.2126 mL	1.0631 mL	2.1262 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.