Carbazole-d8 is the deuterated form of Carbazole (HY-D0204). Carbazole is an aromatic heterocyclic tricyclic. Carbazole inhibits the synthesis of new DNA or RNA and can form novel DNA minor groove complexes.

Physicochemical Properties

Molecular Formula	C12HD8N
Molecular Weight	175.26
Exact Mass	175.123
CAS #	38537-24-5
Related CAS #	Carbazole;86-74-8
PubChem CID	59270898
Appearance	Off-white to light brown solid powder
Density	1.2±0.1 g/cm3
Boiling Point	355.0±11.0 °C at 760 mmHg
Flash Point	160.0±11.9 °C
Vapour Pressure	0.0±0.8 mmHg at 25°C
Index of Refraction	1.768
LogP	3.72
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	0
Rotatable Bond Count	0
Heavy Atom Count	13
Complexity	170
Defined Atom Stereocenter Count	0
SMILES	[2H]C1=C(C(=C2C(=C1[2H])C3=C(C(=C(C(=C3N2)[2H])[2H])[2H])[2H])[2H])[2H]
InChi Key	UJOBWOGCFQCDNV-PGRXLJNUSA-N
InChi Code	InChI=1S/C12H9N/c1-3-7-11-9(5-1)10-6-2-4-8-12(10)13-11/h1-8,13H/i1D,2D,3D,4D,5D,6D,7D,8D
Chemical Name	1,2,3,4,5,6,7,8-octadeuterio-9H-carbazole
Synonyms	Carbazole-d8
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Drug molecules have included stable heavy isotopes of carbon, hydrogen, and other elements, mostly as tracers for quantitation during the drug development process. Due to its potential to alter the pharmacokinetic and metabolic profiles of medications, deuteration has drawn attention[1].
References	[1]. Impact of Deuterium Substitution on the Pharmacokinetics of Pharmaceuticals. Ann Pharmacother. 2019 Feb;53(2):211-216. [2]. A new type of DNA minor-groove complex: carbazole dication-DNA interactions. Biochemistry. 1997 Dec 9;36(49):15315-25.

Solubility Data

Solubility (In Vitro)

DMSO : ~83.33 mg/mL (~475.5 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 2.08 mg/mL (11.87 mM) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.7058 mL	28.5290 mL	57.0581 mL
	5 mM	1.1412 mL	5.7058 mL	11.4116 mL
	10 mM	0.5706 mL	2.8529 mL	5.7058 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.