Calcium hydrogen phosphate (dihydrate), also known as CHPD or DCPD. Dibasic calcium phosphate dihydrate is frequently found in urinary tract stones. Calcium hydrogen phosphate (dihydrate) could be utilized as pharmaceutical excipients, such as diluents and adsorbents. Pharmaceutical excipients or pharmaceutical auxiliaries refer to other chemical substances other than drug ingredients used in the pharmaceutical process. Pharmaceutical excipients generally refer to inactive ingredients in pharmaceutical preparations, which can improve the stability, solubility and processability of pharmaceutical preparations. Pharmaceutical excipients can also affect the absorption, distribution, metabolism, and elimination (ADME) processes of co-administered drugs.

Physicochemical Properties

Molecular Formula	CAH5O6P
Molecular Weight	172.09
Exact Mass	171.944
CAS #	7789-77-7
Related CAS #	10103-46-5 (Parent)
PubChem CID	24441
Appearance	Triclinic crystals White crystalline powder
Density	2.31
Boiling Point	158ºC at 760 mmHg
Melting Point	109°C -H₂O
Index of Refraction	1.5394
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	0
Heavy Atom Count	6
Complexity	46.5
Defined Atom Stereocenter Count	0
SMILES	[Ca+2].P(=O)([O-])([O-])O[H].O([H])[H].O([H])[H]
InChi Key	FUFJGUQYACFECW-UHFFFAOYSA-L
InChi Code	InChI=1S/Ca.H3O4P/c;1-5(2,3)4/h;(H3,1,2,3,4)/q+2;/p-2
Chemical Name	calcium;hydrogen phosphate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ADME/Pharmacokinetics	Absorption, Distribution and Excretion ... ITS ELEMENTS ARE SLOWLY ABSORBED FROM SMALL INTESTINE.
References	[1]. Crystallization and thermal characterization of calcium hydrogen phosphate dihydrate crystals. Journal of thermal analysis and calorimetry, 2009, 96(1): 73-76. [2]. Pharmaceutical excipients - quality, regulatory and biopharmaceutical considerations. Eur J Pharm Sci. 2016 May 25;87:88-99.
Additional Infomation	Calcium hydrogenphosphate is a calcium phosphate. Therapeutic Uses USES--EXCELLENT SOURCE OF CALCIUM & PHOSPHORUS DURING PREGNANCY, LACTATION, OR MILD TO MODERATE HYPOCALCEMIA CHARACTERIZED BY LOW DEGREE OF TETANY. ... IF TETANY IS SEVERE, IV CALCIUM MEDICATION IS ADMIN. ... DOSE--1 TO 5 G/DAY IN 2 OR MORE DIVIDED DOSES. IT IS NOT MARKETED IN SINGLE ENTITY FORM, SO THAT DOSE OF OTHER INGREDIENTS MAY HAVE TO BE CONSIDERED IN ARRIVING @ SUITABLE SCHEDULE. CALCIUM IS BETTER ABSORBED IF TAKEN IN SMALL DOSES @ FREQUENT INTERVALS. MEDICATION (VET): FOR PREVENTION & TREATMENT OF RICKETS ALONG WITH VITAMIN D THERAPY, & AS NUTRIENT SOURCE OF CALCIUM & PHOSPHORUS IN FEEDSTUFFS. ... OPTIMUM CALCIUM:PHOSPHORUS RATIO IN DIET MUST ALSO BE MAINTAINED. DIBASIC CALCIUM PHOSPHATE, NATIONAL FORMULARY ... /IS AMONG CMPD/ CLASSIFIED AS NONSYSTEMIC GASTRIC ANTACIDS, BUT THEIR EFFICACY IS LOW & THEY ARE SELDOM EMPLOYED. Medication (VET): Has been used as a dietary supplement, and as an antacid. Drug Warnings BECAUSE OF PHOSPHATE CONTENT, IT IS CONTRAINDICATED IN /PERSONS WITH/ HYPOPARATHYROIDISM.

Solubility Data

Solubility (In Vitro)

May dissolve in DMSO (in most cases), if not, try other solvents such as H2O, Ethanol, or DMF with a minute amount of products to avoid loss of samples

Solubility (In Vivo)

Note: Listed below are some common formulations that may be used to formulate products with low water solubility (e.g. < 1 mg/mL), you may test these formulations using a minute amount of products to avoid loss of samples. Injection Formulations (e.g. IP/IV/IM/SC) Injection Formulation 1: DMSO : Tween 80： Saline = 10 : 5 : 85 (i.e. 100 μL DMSO stock solution → 50 μL Tween 80 → 850 μL Saline) *Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH ₂ O to obtain a clear solution. Injection Formulation 2: DMSO : PEG300 ：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL DMSO → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Injection Formulation 3: DMSO : Corn oil = 10 : 90 (i.e. 100 μL DMSO → 900 μL Corn oil) Example: Take the Injection Formulation 3 (DMSO : Corn oil = 10 : 90) as an example, if 1 mL of 2.5 mg/mL working solution is to be prepared, you can take 100 μL 25 mg/mL DMSO stock solution and add to 900 μL corn oil, mix well to obtain a clear or suspension solution (2.5 mg/mL, ready for use in animals). Injection Formulation 4: DMSO : 20% SBE-β-CD in saline = 10 : 90 [i.e. 100 μL DMSO → 900 μL (20% SBE-β-CD in saline)] *Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Injection Formulation 5: 2-Hydroxypropyl-β-cyclodextrin : Saline = 50 : 50 (i.e. 500 μL 2-Hydroxypropyl-β-cyclodextrin → 500 μL Saline) Injection Formulation 6: DMSO : PEG300 : castor oil : Saline = 5 : 10 : 20 : 65 (i.e. 50 μL DMSO → 100 μLPEG300 → 200 μL castor oil → 650 μL Saline) Injection Formulation 7: Ethanol : Cremophor : Saline = 10： 10 : 80 (i.e. 100 μL Ethanol → 100 μL Cremophor → 800 μL Saline) Injection Formulation 8: Dissolve in Cremophor/Ethanol (50 : 50), then diluted by Saline Injection Formulation 9: EtOH : Corn oil = 10 : 90 (i.e. 100 μL EtOH → 900 μL Corn oil) Injection Formulation 10: EtOH : PEG300：Tween 80 : Saline = 10 : 40 : 5 : 45 (i.e. 100 μL EtOH → 400 μLPEG300 → 50 μL Tween 80 → 450 μL Saline) Oral Formulations Oral Formulation 1: Suspend in 0.5% CMC Na (carboxymethylcellulose sodium) Oral Formulation 2: Suspend in 0.5% Carboxymethyl cellulose Example: Take the Oral Formulation 1 (Suspend in 0.5% CMC Na) as an example, if 100 mL of 2.5 mg/mL working solution is to be prepared, you can first prepare 0.5% CMC Na solution by measuring 0.5 g CMC Na and dissolve it in 100 mL ddH2O to obtain a clear solution; then add 250 mg of the product to 100 mL 0.5% CMC Na solution, to make the suspension solution (2.5 mg/mL, ready for use in animals). Oral Formulation 3: Dissolved in PEG400 Oral Formulation 4: Suspend in 0.2% Carboxymethyl cellulose Oral Formulation 5: Dissolve in 0.25% Tween 80 and 0.5% Carboxymethyl cellulose Oral Formulation 6: Mixing with food powders Note: Please be aware that the above formulations are for reference only. InvivoChem strongly recommends customers to read literature methods/protocols carefully before determining which formulation you should use for in vivo studies, as different compounds have different solubility properties and have to be formulated differently.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	5.8109 mL	29.0546 mL	58.1091 mL
	5 mM	1.1622 mL	5.8109 mL	11.6218 mL
	10 mM	0.5811 mL	2.9055 mL	5.8109 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.