Calcein tetraethyl ester is a fluorescent dye reagent used in biology because it can enter living cells through the cell membrane and can be used for cell viability detection.

Physicochemical Properties

Molecular Formula	C38H42N2O13
Molecular Weight	734.745691776276
Exact Mass	734.268
CAS #	1170856-93-5
Related CAS #	Calcein;1461-15-0
PubChem CID	91810997
Appearance	Light yellow to yellow solid powder
Density	1.4±0.1 g/cm3
Boiling Point	825.4±65.0 °C at 760 mmHg
Flash Point	453.0±34.3 °C
Vapour Pressure	0.0±3.1 mmHg at 25°C
Index of Refraction	1.638
LogP	6.56
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	15
Rotatable Bond Count	20
Heavy Atom Count	53
Complexity	1180
Defined Atom Stereocenter Count	0
InChi Key	AWKMLDYACNCHOZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C38H42N2O13/c1-5-48-31(43)19-39(20-32(44)49-6-2)17-24-29(41)15-13-27-35(24)52-36-25(18-40(21-33(45)50-7-3)22-34(46)51-8-4)30(42)16-14-28(36)38(27)26-12-10-9-11-23(26)37(47)53-38/h9-16,41-42H,5-8,17-22H2,1-4H3
Chemical Name	ethyl 2-[[5'-[[bis(2-ethoxy-2-oxoethyl)amino]methyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl]methyl-(2-ethoxy-2-oxoethyl)amino]acetate
Synonyms	Calcein tetraethyl ester; 1170856-93-5; Calcein (tetraethyl ester); Fluorexon tetraethyl ester; tetraethyl 2,2',2'',2'''-(((3',6'-dihydroxy-3-oxo-3H-spiro[isobenzofuran-1,9'-xanthene]-4',5'-diyl)bis(methylene))bis(azanetriyl))tetraacetate; ethyl 2-[[5'-[[bis(2-ethoxy-2-oxoethyl)amino]methyl]-3',6'-dihydroxy-3-oxospiro[2-benzofuran-1,9'-xanthene]-4'-yl]methyl-(2-ethoxy-2-oxoethyl)amino]acetate; Glycine, N,N'-[(3',6'-dihydroxy-3-oxospiro[isobenzofuran-1(3H),9'-[9H]xanthene]-4',5'-diyl)bis(methylene)]bis[N-(2-ethoxy-2-oxoethyl)-, 1,1'-diethyl ester; Tetraethyl Fluorescein Bis(methylene)bis(azanetriyl)tetraacetate;
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets

Fluorescent Dye; Emission (Em) = 515; Excitation (Ex) = 419

Solubility Data

Solubility (In Vitro)

DMSO : ~25 mg/mL (~34.03 mM) H2O : ~0.67 mg/mL (~0.91 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.5 mg/mL (3.40 mM) (saturation unknown) in 10% DMSO + 40% PEG300 +5% Tween-80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 25.0 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 + to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.3610 mL	6.8050 mL	13.6101 mL
	5 mM	0.2722 mL	1.3610 mL	2.7220 mL
	10 mM	0.1361 mL	0.6805 mL	1.3610 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.