Cy5-SE (Cy5 NHS Ester) triethylamine salt is a reactive dye used to label the amino groups of peptides, proteins, and oligonucleotides (Ex=649 nm; Em=670 nm).

Physicochemical Properties

Molecular Formula	C43H58N4O10S2
Molecular Weight	855.07
Exact Mass	854.359
CAS #	1497420-70-8
Related CAS #	CY5-SE;146368-14-1
PubChem CID	154728096
Appearance	Purple to purplish red solid powder
Hydrogen Bond Donor Count	1
Hydrogen Bond Acceptor Count	12
Rotatable Bond Count	16
Heavy Atom Count	59
Complexity	1750
Defined Atom Stereocenter Count	0
SMILES	CCN\1C2=C(C=C(C=C2)S(=O)(=O)[O-])C(/C1=C\C=C\C=C\C3=[N+](C4=C(C3(C)C)C=C(C=C4)S(=O)(=O)O)CCCCCC(=O)ON5C(=O)CCC5=O)(C)C.CCN(CC)CC
InChi Key	IFUMHFCOWWJPSC-UHFFFAOYSA-N
InChi Code	InChI=1S/C37H43N3O10S2.C6H15N/c1-6-38-29-18-16-25(51(44,45)46)23-27(29)36(2,3)31(38)13-9-7-10-14-32-37(4,5)28-24-26(52(47,48)49)17-19-30(28)39(32)22-12-8-11-15-35(43)50-40-33(41)20-21-34(40)42;1-4-7(5-2)6-3/h7,9-10,13-14,16-19,23-24H,6,8,11-12,15,20-22H2,1-5H3,(H-,44,45,46,47,48,49);4-6H2,1-3H3
Chemical Name	N,N-diethylethanamine;(2E)-2-[(2E,4E)-5-[1-[6-(2,5-dioxopyrrolidin-1-yl)oxy-6-oxohexyl]-3,3-dimethyl-5-sulfoindol-1-ium-2-yl]penta-2,4-dienylidene]-1-ethyl-3,3-dimethylindole-5-sulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Advice (This is our suggested protocol, which should be adjusted to suit your particular circumstances as it simply offers guidance). SELP analogues conjugated to Cy5-SE. 1. Combine 10 μL of 1.2 mg/mL Cy5 monoNHS-ester in DMSO with 1 mL of 100 μg/mL SELP solution in 100 mM sodium bicarbonate buffer (pH 8.3), then let it sit on ice for two hours. ..2. To quench the reaction, add 50 μL of 1 M Tris-HCl (pH 8.0) to the reaction solution. 3. Centrifugation at 1050g[1] for three minutes is the method used to elute the Cy5-conjugated SELP after loading the reaction mixture onto a 1.5 mL Sephadex G-25 column.

References

[1]. Array-Based High-Throughput Analysis of Silk-Elastinlike Protein Polymer Degradation and C-Peptide Release by Proteases. Anal Chem. 2016;88(10):5398-5405.

Solubility Data

Solubility (In Vitro)

DMSO: 125 mg/mL (146.19 mM) H2O: ≥ 5.88 mg/mL (6.88 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (2.43 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: 25 mg/mL (29.24 mM) in Saline (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication (<60°C). Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.1695 mL	5.8475 mL	11.6949 mL
	5 mM	0.2339 mL	1.1695 mL	2.3390 mL
	10 mM	0.1169 mL	0.5847 mL	1.1695 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.