CTX-0294885 HCl is a broad-spectrum kinase inhibitor that captures 235 kinases from MDA-MB-231 cells and captures all members of the AKT family. CTX-0294885 HCl is a potent reagent for kinome signaling network analysis and may be utilized to study diseases such as inflammation, diabetes, and cancer.

Physicochemical Properties

Molecular Formula	C22H25CL2N7O
Molecular Weight	474.39
Related CAS #	CTX-0294885;1439934-41-4
Appearance	Light yellow to yellow solid powder
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro

Large-scale kinome profiling research using CTX-0294885 hydrochloride as a kinase capture method have discovered 185 kinases as total protein and 179 as phosphopeptides from TiO2 enrichment in MDA-MB-231 cells (by quantitative MS)[1]. All members of the AKT family that have not been identified by earlier research employing alternative kinase capture reagents are captured by CTX -0294885 hydrochloride[1].

References

[1]. Characterization of the novel broad-spectrum kinase inhibitor CTx-0294885 as an affinity reagent for mass spectrometry-based kinome profiling. J Proteome Res. 2013 Jul 5;12(7):3104-16.

Solubility Data

Solubility (In Vitro)

H2O :~55 mg/mL (~115.94 mM) DMSO :~9.17 mg/mL (~19.33 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 0.92 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.2 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 0.92 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.2 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 3: ≥ 0.92 mg/mL (1.94 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 9.2 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly. Solubility in Formulation 4: 50 mg/mL (105.40 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with ultrasonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1080 mL	10.5399 mL	21.0797 mL
	5 mM	0.4216 mL	2.1080 mL	4.2159 mL
	10 mM	0.2108 mL	1.0540 mL	2.1080 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.