TAO Kinase inhibitor 1 (compound 43) is a selective, ATP-competitive inhibitor of thousandsand-and-one amino acid (AA) kinases (TAOK) with IC50s of 11 nM and 15 nM for TAOK1 and TAOK2, respectively. TAO Kinase inhibitor 1 delays mitosis and induces mitotic cell death.

Physicochemical Properties

Molecular Formula	C25H24N2O2
Molecular Weight	384.479
Exact Mass	384.183
CAS #	850467-66-2
PubChem CID	46673962
Appearance	White to off-white solid powder
Density	1.2±0.1 g/cm3
Boiling Point	673.2±54.0 °C at 760 mmHg
Flash Point	224.7±31.4 °C
Vapour Pressure	0.0±2.1 mmHg at 25°C
Index of Refraction	1.647
LogP	5.37
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	2
Rotatable Bond Count	5
Heavy Atom Count	29
Complexity	549
Defined Atom Stereocenter Count	0
InChi Key	WQKXOAJVNFOHNZ-UHFFFAOYSA-N
InChi Code	InChI=1S/C25H24N2O2/c28-24(27-23-12-6-10-20-9-4-5-11-22(20)23)17-26-25(29)21-15-13-19(14-16-21)18-7-2-1-3-8-18/h1-5,7-9,11,13-16,23H,6,10,12,17H2,(H,26,29)(H,27,28)
Chemical Name	N-[2-oxo-2-(1,2,3,4-tetrahydronaphthalen-1-ylamino)ethyl]-4-phenylbenzamide
Synonyms	CP-43; CP43; CP 43
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	Centrosome-amplified breast cancer cells undergo mitotic cell death and mitosis delay when exposed to TAO kinase inhibitor 1 [1].
References	[1]. Targeting TAO Kinases Using a New Inhibitor Compound Delays Mitosis and Induces Mitotic Cell Death in Centrosome Amplified Breast Cancer Cells. Mol Cancer Ther. 2017 Nov;16(11):2410-2421.

Solubility Data

Solubility (In Vitro)

DMSO : ~62.5 mg/mL (~162.56 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 2.08 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 2.08 mg/mL (5.41 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 20.8 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.6009 mL	13.0046 mL	26.0092 mL
	5 mM	0.5202 mL	2.6009 mL	5.2018 mL
	10 mM	0.2601 mL	1.3005 mL	2.6009 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.