Green CMFDA (CMFDA) is a cell-penetrating/penetrable fluorescent probe that can be used as a cell tracer. Green CMFDA can be cleaved by non-specific esterases common in living cells, producing a fluorescent compound, fluorescein, visible under a fluorescence microscope.

Physicochemical Properties

Molecular Formula	C25H17O7CL
Molecular Weight	464.85128
Exact Mass	464.066
CAS #	136832-63-8
PubChem CID	122297
Appearance	White to off-white solid powder
Density	1.49 g/cm3
Boiling Point	657.5ºC at 760 mmHg
Flash Point	238.3ºC
Vapour Pressure	3.65E-17mmHg at 25°C
Index of Refraction	1.675
LogP	4
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	5
Heavy Atom Count	33
Complexity	760
Defined Atom Stereocenter Count	0
SMILES	CC(=O)OC1=CC2=C(C=C1)C3(C4=C(C=C(C=C4)CCl)C(=O)O3)C5=C(C=C(C=C5)OC(=O)C)O2
InChi Key	IPJDHSYCSQAODE-UHFFFAOYSA-N
InChi Code	InChI=1S/C25H17ClO7/c1-13(27)30-16-4-7-20-22(10-16)32-23-11-17(31-14(2)28)5-8-21(23)25(20)19-6-3-15(12-26)9-18(19)24(29)33-25/h3-11H,12H2,1-2H3
Chemical Name	[6'-acetyloxy-5-(chloromethyl)-3-oxospiro[2-benzofuran-1,9'-xanthene]-3'-yl] acetate
Synonyms	CM-FDA; Cell Tracker Green; CMFDA
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: This product requires protection from light (avoid light exposure) during transportation and storage.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

References	[1]. Challenges in using CellTracker Green on foraminifers that host algal endosymbionts. PeerJ. 2018 Aug 30;6:e5304.
Additional Infomation	5-chloromethylfluorescein diacetate is an acetate ester. It has a role as a fluorochrome. It is functionally related to a fluorescein (lactone form).

Solubility Data

Solubility (In Vitro)

DMSO : ~33.33 mg/mL (~71.70 mM)

Solubility (In Vivo)

Solubility in Formulation 1: ≥ 1.43 mg/mL (3.08 mM) (saturation unknown) in 10% DMSO + 40% PEG300 + 5% Tween80 + 45% Saline (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 400 μL PEG300 and mix evenly; then add 50 μL Tween-80 to the above solution and mix evenly; then add 450 μL normal saline to adjust the volume to 1 mL. Preparation of saline: Dissolve 0.9 g of sodium chloride in 100 mL ddH₂ O to obtain a clear solution. Solubility in Formulation 2: ≥ 1.43 mg/mL (3.08 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 14.3 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1512 mL	10.7562 mL	21.5123 mL
	5 mM	0.4302 mL	2.1512 mL	4.3025 mL
	10 mM	0.2151 mL	1.0756 mL	2.1512 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.