CLinDMA is a cationic lipid that induces an inflammatory response. CLinDMA may be utilized to prepare LNP201. LNP201 is a liposome component for systemic delivery of siRNA.

Physicochemical Properties

Molecular Formula	C54H97NO3
Molecular Weight	808.352897405624
CAS #	908860-82-2
PubChem CID	70876560
Appearance	Light yellow to yellow viscous liquid
Hydrogen Bond Donor Count	0
Hydrogen Bond Acceptor Count	4
Rotatable Bond Count	31
Heavy Atom Count	58
Complexity	1170
Defined Atom Stereocenter Count	8
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment (e.g. under nitrogen), avoid exposure to moisture and light.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	LNP201 uses an RNA interference mechanism to cause long-term silencing of the mouse Ssb gene in mouse livers following one or more doses. In addition to inducing the expression of many genes involved in innate immunity and mitogen-activated protein kinase (MAPK) activation in tissues, LNP201 generates an acute inflammatory response [1].
References	[1]. Evaluation of efficacy, biodistribution, and inflammation for a potent siRNA nanoparticle: effect of dexamethasone co-treatment. Mol Ther. 2010;18(1):171-180.

Solubility Data

Solubility (In Vitro)

DMSO : ~140 mg/mL (~173.19 mM)

Solubility (In Vivo)

Solubility in Formulation 1: 3.5 mg/mL (4.33 mM) in 10% DMSO + 90% (20% SBE-β-CD in Saline) (add these co-solvents sequentially from left to right, and one by one), suspension solution; with sonication. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 35.0 mg/mL clear DMSO stock solution to 900 μL of 20% SBE-β-CD physiological saline solution and mix evenly. Preparation of 20% SBE-β-CD in Saline (4°C，1 week): Dissolve 2 g SBE-β-CD in 10 mL saline to obtain a clear solution. Solubility in Formulation 2: ≥ 3.5 mg/mL (4.33 mM) (saturation unknown) in 10% DMSO + 90% Corn Oil (add these co-solvents sequentially from left to right, and one by one), clear solution. For example, if 1 mL of working solution is to be prepared, you can add 100 μL of 35.0 mg/mL clear DMSO stock solution to 900 μL of corn oil and mix evenly.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.2371 mL	6.1854 mL	12.3709 mL
	5 mM	0.2474 mL	1.2371 mL	2.4742 mL
	10 mM	0.1237 mL	0.6185 mL	1.2371 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.