CL-316243 disodium (CL316243) is a novel and potent β3-adrenoceptor agonist with the potential for the treatment obesity, diabetes and urge urinary incontinence. It activates β3-adrenoceptor with a EC50 of 3 nM.

Physicochemical Properties

Molecular Formula	C20H18CLNNA2O7
Molecular Weight	465.7935
Exact Mass	465.057
CAS #	138908-40-4
PubChem CID	5312115
Appearance	White to yellow solid powder
Boiling Point	689.5ºC at 760 mmHg
Flash Point	370.8ºC
Vapour Pressure	5.86E-20mmHg at 25°C
Hydrogen Bond Donor Count	2
Hydrogen Bond Acceptor Count	8
Rotatable Bond Count	6
Heavy Atom Count	31
Complexity	578
Defined Atom Stereocenter Count	2
SMILES	C[C@H](CC1=CC2=C(C=C1)OC(O2)(C(=O)[O-])C(=O)[O-])NC[C@@H](C3=CC(=CC=C3)Cl)O.[Na+].[Na+]
InChi Key	FUZBPOHHSBDTJQ-CFOQQKEYSA-L
InChi Code	InChI=1S/C20H20ClNO7.2Na/c1-11(22-10-15(23)13-3-2-4-14(21)9-13)7-12-5-6-16-17(8-12)29-20(28-16,18(24)25)19(26)27;;/h2-6,8-9,11,15,22-23H,7,10H2,1H3,(H,24,25)(H,26,27);;/q;2*+1/p-2/t11-,15+;;/m1../s1
Chemical Name	disodium;5-[(2R)-2-[[(2R)-2-(3-chlorophenyl)-2-hydroxyethyl]amino]propyl]-1,3-benzodioxole-2,2-dicarboxylate
Synonyms	CL316243 CL 316243 CL-316243
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month Note: Please store this product in a sealed and protected environment, avoid exposure to moisture.
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

Targets	EC50: 3 nM (β3-adrenoceptor)[1]
ln Vitro	Clenidine 316243 has IC50 values of 0.6 μM and 1 μM, respectively, against the rat heart and soleus muscle[1]. Based on concentration-dependent inhibition, CL 316243 prevents 50% of the maximal response from spontaneous contractions in isolated rat detrusor muscle strips at an average dose of 2.65 nM [3].
ln Vivo	Unaffected by food, CL316243 disodium (subcutaneous injection; 0.1 mg/kg/d; once daily; 1 week) raises BAT's levels of UCP1 mRNA and protein expression [2].
Animal Protocol	Animal/Disease Models: Male C57BL/6J mice fed with high-fat diets (HFD; 45%-kcal fat) or a control diet (ND; 10%-kcal fat) for 14 weeks[2] Doses: 0.1 mg/kg/day Route of Administration: one time/day; 1 weeks Experimental Results: demonstrated a premium effect of obesity in mice.
References	[1]. Disodium (R,R)-5-[2-[[2-(3-chlorophenyl)-2-hydroxyethyl]-amino] propyl]-1,3-benzodioxole-2,2-dicarboxylate (CL 316,243). A potent beta-adrenergic agonist virtually specific for beta 3 receptors. A promising antidiabetic and antiobesity agent.J Med Chem. 1992 Aug 7;35(16):3081-4. [2]. Impaired adrenergic agonist-dependent beige adipocyte induction in obese mice.J Vet Med Sci. 2019 Jun 6;81(6):799-807.

Solubility Data

Solubility (In Vitro)	H2O : ~20 mg/mL (~42.94 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 100 mg/mL (214.69 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication (<60°C).  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	2.1469 mL	10.7345 mL	21.4689 mL
	5 mM	0.4294 mL	2.1469 mL	4.2938 mL
	10 mM	0.2147 mL	1.0734 mL	2.1469 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.