CHAPS, an analogue of Cholic acid, is a zwitterionic detergent that solubilizes membrane proteins. CHAPS are used to stabilize a variety of protein-DNA complexes and can preserve the biochemical activity of proteins in solution.

Physicochemical Properties

Molecular Formula	C32H58N2O7S
Molecular Weight	614.8771
Exact Mass	614.396
CAS #	75621-03-3
Related CAS #	CHAPS hydrate;331717-45-4
PubChem CID	107670
Appearance	White to off-white solid powder
Density	1.01 g/mL at 20ºC
Melting Point	156-158ºC
Index of Refraction	1.6900 (estimate)
LogP	-4.32
Hydrogen Bond Donor Count	4
Hydrogen Bond Acceptor Count	7
Rotatable Bond Count	11
Heavy Atom Count	42
Complexity	1030
Defined Atom Stereocenter Count	11
SMILES	C[C@H](CCC(=O)NCCC[N+](C)(C)CCCS(=O)(=O)[O-])[C@H]1CC[C@@H]2[C@@]1([C@H](C[C@H]3[C@H]2[C@@H](C[C@H]4[C@@]3(CC[C@H](C4)O)C)O)O)C
InChi Key	UMCMPZBLKLEWAF-BCTGSCMUSA-N
InChi Code	InChI=1S/C32H58N2O7S/c1-21(8-11-29(38)33-14-6-15-34(4,5)16-7-17-42(39,40)41)24-9-10-25-30-26(20-28(37)32(24,25)3)31(2)13-12-23(35)18-22(31)19-27(30)36/h21-28,30,35-37H,6-20H2,1-5H3,(H-,33,38,39,40,41)/t21-,22+,23-,24-,25+,26+,27-,28+,30+,31+,32-/m1/s1
Chemical Name	3-[dimethyl-[3-[[(4R)-4-[(3R,5S,7R,8R,9S,10S,12S,13R,14S,17R)-3,7,12-trihydroxy-10,13-dimethyl-2,3,4,5,6,7,8,9,11,12,14,15,16,17-tetradecahydro-1H-cyclopenta[a]phenanthren-17-yl]pentanoyl]amino]propyl]azaniumyl]propane-1-sulfonate
HS Tariff Code	2934.99.9001
Storage	Powder-20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Shipping Condition	Room temperature (This product is stable at ambient temperature for a few days during ordinary shipping and time spent in Customs)

Biological Activity

ln Vitro	CHAPS (0.5 %) can maintain the complex between DNA and DNA-binding factors (such as AP-1, SPI, GATA-1 and alpha regulatory factor ISGF3) and retain its biological activity [2]. CHAPS suppresses mononucleosome dissociation at subnanomolar doses of cytokines [2].
References	[1]. Thermodynamic characterization of 3-[(3-cholamidopropyl)-dimethylammonium]-1-propanesulfonate (CHAPS) micellization using isothermal titration calorimetry: temperature, salt, and pH dependence. Langmuir. 2012 Jul 17;28(28):10363-71. [2]. Nucleosomes structure and dynamics: effect of CHAPS. Int J Biochem Mol Biol. 2011;2(2):129-137.
Additional Infomation	3-[(3-cholamidopropyl)dimethylammonio]propane-1-sulfonate is an ammonium betaine. It is functionally related to a cholic acid. It is a conjugate base of a 3-[(cholamidopropyl)dimethylammonio]-1-propanesulfonate.

Solubility Data

Solubility (In Vitro)	H2O : ~83.33 mg/mL (~135.52 mM)
Solubility (In Vivo)	Solubility in Formulation 1: 10 mg/mL (16.26 mM) in PBS (add these co-solvents sequentially from left to right, and one by one), clear solution; with sonication.  (Please use freshly prepared in vivo formulations for optimal results.)

Preparing Stock Solutions		1 mg	5 mg	10 mg
	1 mM	1.6263 mL	8.1317 mL	16.2633 mL
	5 mM	0.3253 mL	1.6263 mL	3.2527 mL
	10 mM	0.1626 mL	0.8132 mL	1.6263 mL

*Note: Please select an appropriate solvent for the preparation of stock solution based on your experiment needs. For most products, DMSO can be used for preparing stock solutions (e.g. 5 mM, 10 mM, or 20 mM concentration); some products with high aqueous solubility may be dissolved in water directly. Solubility information is available at the above Solubility Data section. Once the stock solution is prepared, aliquot it to routine usage volumes and store at -20°C or -80°C. Avoid repeated freeze and thaw cycles.